LOCUS       V043190                11829 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043190
VERSION     V043190
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5931
                     /label="Egfr(NM_207655)"
                     /note="Egfr(NM_207655)"
                     /gene="Egfr"
     CDS             5938..6003
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6050..6261
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6274..6542
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6577..7269
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             7270..7323
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7324..7923
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7924..8512
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8586..8819
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8891..9012
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9031..9166
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9204..9220)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9228..9244
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9252..9282)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9297..9318
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9606..10194)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10365..11225)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11226..11330)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11804..11820
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcgaccc tcagggaccg cgagaaccac actgctggtg
     2341 ttgctgaccg cgctctgcgc cgcaggtggg gcgttggagg aaaagaaagt ctgccaaggc
     2401 acaagtaaca ggctcaccca actgggcact tttgaagacc actttctgag cctgcagagg
     2461 atgtacaaca actgtgaagt ggtccttggg aacttggaaa ttacctatgt gcaaaggaat
     2521 tacgaccttt ccttcttaaa gaccatccag gaggtggccg gctatgtcct cattgccctc
     2581 aacaccgtgg agagaatccc tttggagaac ctgcagatca tcaggggaaa tgctctttat
     2641 gaaaacacct atgccttagc catcctgtcc aactatggga caaacagaac tgggcttagg
     2701 gaactgccca tgcggaactt acaggaaatc ctgattggtg ctgtgcgatt cagcaacaac
     2761 cccatcctct gcaatatgga tactatccag tggagggaca tcgtccaaaa cgtctttatg
     2821 agcaacatgt caatggactt acagagccat ccgagcagtt gccccaaatg tgatccaagc
     2881 tgtcccaatg gaagctgctg gggaggagga gaggagaact gccagaaatt gaccaaaatc
     2941 atctgtgccc agcaatgttc ccatcgctgt cgtggcaggt cccccagtga ctgctgccac
     3001 aaccaatgtg ctgcggggtg tacagggccc cgagagagtg actgtctggt ctgccaaaag
     3061 ttccaagatg aggccacatg caaagacacc tgcccaccac tcatgctgta caaccccacc
     3121 acctatcaga tggatgtcaa ccctgaaggg aagtacagct ttggtgccac ctgtgtgaag
     3181 aagtgccccc gaaactacgt ggtgacagat catggctcat gtgtccgagc ctgtgggcct
     3241 gactactacg aagtggaaga agatggcatc cgcaagtgta aaaaatgtga tgggccctgt
     3301 cgcaaagttt gtaatggcat aggcattggt gaatttaaag acacactctc cataaatgct
     3361 acaaacatca aacacttcaa atactgcact gccatcagcg gggaccttca catcctgcca
     3421 gtggccttta agggggattc tttcacgcgc actcctcctc tagacccacg agaactagaa
     3481 attctaaaaa ccgtaaagga aataacaggc tttttgctga ttcaggcttg gcctgataac
     3541 tggactgacc tccatgcttt cgagaaccta gaaataatac gtggcagaac aaagcaacat
     3601 ggtcagtttt ctttggcggt cgttggcctg aacatcacat cactggggct gcgttccctc
     3661 aaggagatca gtgatgggga tgtgatcatt tctggaaacc gaaatttgtg ctacgcaaac
     3721 acaataaact ggaaaaaact cttcgggaca cccaatcaga aaaccaaaat catgaacaac
     3781 agagctgaga aagactgcaa ggccgtgaac cacgtctgca atcctttatg ctcctcggaa
     3841 ggctgctggg gccctgagcc cagggactgt gtctcctgcc agaatgtgag cagaggcagg
     3901 gagtgcgtgg agaaatgcaa catcctggag ggggaaccaa gggagtttgt ggaaaattct
     3961 gaatgcatcc agtgccatcc agaatgtctg ccccaggcca tgaacatcac ctgtacaggc
     4021 aggggaccag acaactgcat ccagtgtgcc cactacattg atggcccaca ctgtgtcaag
     4081 acctgcccag ctggcatcat gggagagaac aacactctgg tctggaagta tgcagatgcc
     4141 aataatgtct gccacctatg ccacgccaac tgtacctatg gatgtgctgg gccaggtctt
     4201 caaggatgtg aagtgtggcc atctgggcca aagataccat ctattgccac tgggattgtg
     4261 ggtggcctcc tcttcatagt ggtggtggcc cttgggattg gcctattcat gcgaagacgt
     4321 cacattgttc gaaagcgtac actacgccgc ctgcttcaag agagagagct cgtggaacct
     4381 ctcacaccca gcggagaagc tccaaaccaa gcccacttga ggatattaaa ggaaacagaa
     4441 ttcaaaaaga tcaaagttct gggttcggga gcatttggca cagtgtataa gggtctctgg
     4501 atcccagaag gtgagaaagt aaaaatcccg gtggccatca aggagttaag agaagccaca
     4561 tctccaaaag ccaacaaaga aatccttgac gaagcctatg tgatggctag tgtggacaac
     4621 cctcatgtat gccgcctcct gggcatctgt ctgacctcca ctgtccagct cattacacag
     4681 ctcatgccct acggttgcct cctggactac gtccgagaac acaaggacaa cattggctcc
     4741 cagtacctcc tcaactggtg tgtgcagatt gcaaagggca tgaactacct ggaagatcgg
     4801 cgtttggtgc accgtgactt ggcagccagg aatgtactgg tgaagacacc acagcatgtc
     4861 aagatcacag attttgggct ggccaaactg cttggtgctg aagagaaaga atatcatgcc
     4921 gaggggggca aagtgcctat caagtggatg gctttggaat caattttaca ccgaatttat
     4981 acacaccaaa gtgatgtctg gagctatggt gtcactgtgt gggaactgat gacctttggg
     5041 tccaagcctt atgatggaat cccagcaagt gacatctcat ccatcctaga gaaaggagag
     5101 cgccttccac agccacctat ctgcaccatc gatgtctaca tgatcatggt caagtgctgg
     5161 atgatagatg ctgatagccg cccaaagttc cgagagttga ttcttgaatt ctccaaaatg
     5221 gcccgagacc cacagcgcta ccttgttatc cagggggatg aaagaatgca tttgccaagc
     5281 cctacagact ccaactttta ccgagccctg atggatgaag aggacatgga ggatgtagtt
     5341 gatgctgatg agtatcttat cccacagcaa ggcttcttca acagcccgtc cacgtcgagg
     5401 actcccctct tgagttctct gagtgcaact agcaacaatt ccactgtggc ttgcattaat
     5461 agaaatggga gctgccgtgt caaagaagac gccttcttgc agcggtacag ctccgacccc
     5521 acaggtgctg taacagagga caacatagat gacgcattcc tccctgtacc tgaatatgta
     5581 aaccaatctg ttcccaagag gccagcaggc tctgtgcaga accctgtcta tcacaatcag
     5641 cccctgcatc cagctcctgg aagagacctg cattatcaaa atccccacag caatgcagtg
     5701 ggcaaccctg agtatctcaa cactgcccag cctacctgtc tcagtagtgg gtttaacagc
     5761 cctgcactct ggatccagaa aggcagtcac caaatgagcc tagacaaccc tgactaccag
     5821 caggacttct tccccaagga aaccaagcca aatggcatat ttaagggccc cacagctgaa
     5881 aatgcagagt acctacgggt ggcacctcca agcagtgagt ttattggagc aggatccgac
     5941 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     6001 aagtagtgag cggccgcaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     6061 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     6121 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     6181 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     6241 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     6301 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     6361 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     6421 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     6481 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     6541 tccaagctgt gaccggcgcc tacgctagac gccaccatga gcgagctgat caaggagaac
     6601 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
     6661 gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt ggtcgagggc
     6721 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaagcc
     6781 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
     6841 acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     6901 agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa cttcccatcc
     6961 aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga gatgctgtac
     7021 cccgctgacg gcggcctgag aggccacagc cagatggccc tgaagctcgt gggcgggggc
     7081 tacctgcact gctccttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
     7141 atgcccggct tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag
     7201 acctacgtcg agcagcacga gatggctgtg gccaagtact gcgacctccc tagcaaactg
     7261 gggcacagag agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     7321 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     7381 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     7441 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     7501 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     7561 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     7621 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     7681 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     7741 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     7801 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7861 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7921 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7981 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     8041 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     8101 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     8161 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     8221 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     8281 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     8341 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8401 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8461 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     8521 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8581 gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg
     8641 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8701 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8761 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8821 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8881 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8941 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9001 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc
     9061 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9121 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     9181 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9241 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9301 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9361 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9421 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9481 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9541 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9601 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9661 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9721 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9781 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9841 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9901 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9961 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10021 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    10081 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10141 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10201 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10261 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10321 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10381 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10441 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10501 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10561 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10621 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10681 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10741 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10801 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10861 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10921 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10981 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11041 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11101 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11161 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11221 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11281 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11341 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    11401 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
    11461 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    11521 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
    11581 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    11641 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
    11701 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    11761 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    11821 gccaagctg
//
