LOCUS       V033197                10321 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033197
VERSION     V033197
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5008
                     /label="E2f7(NM_001358560)"
                     /note="E2f7(NM_001358560)"
                     /gene="E2f7"
     CDS             5015..5080
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5124..5335
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5348..5616
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5645..5654
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5675..6337
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     misc_feature    6416..7004
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7078..7311
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7383..7504
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7523..7658
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7696..7712)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7720..7736
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7744..7774)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7789..7810
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8098..8686)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8857..9717)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9718..9822)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10296..10312
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg aggtgaattg tttaacacta aaagacttga tcagccccag
     2341 gcagaccaga ctagattttg ccattgaaga tgcagagaac gcacaaaagg aaaatatatt
     2401 tgtcgaccga tcaaggatga ccccaaagac accgatgaag aacgagccga tcgacctgtc
     2461 aaagcaaaga atcttcaccc cagacagaaa ccccattact ccagtaaagc cggtcgacag
     2521 gcagccgcag gtggagccct ggacacccac agccaacctg aagatgctca tcagcgccgc
     2581 cagcccagac ataagagacc gggagaagaa aaaggagctg ttcagaccca ttgagaataa
     2641 ggaggatgcg ttcgtgaact ccctgcagct tgacgtggcc ggtgacggcg ctgtggatga
     2701 gtatgagaag caaaggccaa gcaggaaaca gaagagcttg gggctgctgt gccagaagtt
     2761 tctagctcgc tatccgagct accccttgtc aacggagaaa accaccatct ccctagatga
     2821 ggtcgccgtc agtctcggcg tggaaaggag acgcatctat gacatcgtaa acgtgctgga
     2881 gtcgctgcat ctggtcagcc gggtggctaa gaatcagtat ggttggcacg gtcggcacag
     2941 cctccccaaa accctgagga ccctacagag actgggagag gaacagaaat acgaggagca
     3001 gatggcctgc ctccagcaga aggagctgga cctgatgggg tataggttcg gagaacgcag
     3061 gaaagacggg tctccggacc cccgagatcc acacctactt gatttttccg aagccgacta
     3121 cccctcttca tctgcaaaca gtcgaaaaga taaatcttta agaattatga gccagaagtt
     3181 tgtcatgctg ttcctcgtct ccaaaaccaa gattgtcacc ctggatgtag ctgccaaaat
     3241 cctcatagaa gaaagccaag ataccccgga ccacagcaaa ttcaaaacaa aggtacggag
     3301 gctctatgac atagccaatg ttctgaccag cctggctctg ataaagaaag ttcacgtaac
     3361 agaagagcga ggccggaaac cagccttcaa gtggatcggg cctgtggact tcagctccat
     3421 tgatgaagaa ctgttggacg tctctgcatc catcttacca gaattgaaga aggaagcata
     3481 cggccagatc cgagtgtgtg caaaagagag gctggtgcgg tacggttcct ttaacacggt
     3541 tcacacgtct gagaagatac agagaaaagt gagctcagaa cccagcagcc cacagggaga
     3601 aagacaaggg tcagcctatt ccctggaaat tggaagtctg gcggccatct acagacagaa
     3661 agtagaagat aattcgcagg aggaagcctt tgtcagtaac acagcagtgc ctccagcaag
     3721 catcttggac cccgccctct ccatggactc tgaatactgt gtcaagcctt tagcccagcc
     3781 agtattttct gttgctcaaa cagatttgcc ggccttctct gcgcagaatg gtccaagcgg
     3841 acaagtaggt gtccccgttc cttctgcagc ctccgacaca gagaacttaa agccagctct
     3901 gctcgccggc cagcccctgg tctacgtgcc ctccacccaa ctcttcatgc tgtacgggag
     3961 tgtgcaggag ggactgtccc cagagtcaag gtcagaggaa gatggtggtg gctccgatgt
     4021 cccagctgac ctgtcagtga caccctctgc tcagaagcgc ctgtgtgagg agagagatcc
     4081 tcaggaggag gaggacgagc ccgccatgaa aagacaaagt caggaatttg aagacagccc
     4141 cttatcccta gttatgccca agaaaccctc aagttccaca gaccttgcct gtcctgtgac
     4201 catgggaaac gggagctctc cacccctgga agacgcctgt gttaagggtc agctccctgc
     4261 tgcagaagaa gttacaggaa aggctgcccc aaactgctat gttgcttctg agtgtggaaa
     4321 cccggcaaga aatccagaca ctgaaaagcc ttccaacgaa aacgagatca ccaaagaccc
     4381 ctcgctgatg cagtacctgt atgtgcagtc tcccgcaggg ttaaatggtt ttaacatggt
     4441 tttaccaggc acccagaccc cccacaccgt ggcaccctcc ccggctcagc tgccatcctt
     4501 cggcgtaccc tgcatgttcc tgcaatcccc aggcctgggc ccttttccag ttctctattc
     4561 tcccgcaata cccgggccca tttcttcagc ccccggcact cacccaaacc ccggacccat
     4621 gaacttcggc ttgtccaccc tggcatctgc ttctcacctt ctcatcagcc cggcagccat
     4681 ggtcaatcca aagccatcca ccctcccctg tacagaccct cagcttcgct gtcagccctc
     4741 actcaacctg aaccccgtga tgccaggctc acacggcgtc atccaccccg agtccccctg
     4801 ttacgtgaga catccggtat cgatggtaaa ggcagagcag tcccctgcac cggcgactcc
     4861 caagagcatc caacggagac atcgggagac gttcttcaag actcccggca gcctcggcga
     4921 tccggtcttt agaagaaaag aaaggaacca atcaagaaac actagctcag cccagaggag
     4981 actagaaatc tctagcagcg ggcctgacgg atccgactac aaagaccatg acggtgatta
     5041 taaagatcat gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg
     5101 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     5161 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     5221 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     5281 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     5341 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     5401 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     5461 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     5521 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     5581 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     5641 agacgccacc atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat
     5701 caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa
     5761 gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta
     5821 cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta
     5881 cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa
     5941 gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt
     6001 gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga
     6061 caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct
     6121 ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt
     6181 ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc
     6241 catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga
     6301 gtaccagcac gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa
     6361 ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgctaag tcgacaatca
     6421 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6481 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6541 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6601 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6661 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6721 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6781 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6841 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6901 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6961 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac
     7021 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7081 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct
     7141 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7201 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7261 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7321 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7381 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7441 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7501 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     7561 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7621 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
     7681 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8521 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8821 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8881 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8941 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9001 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9061 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9841 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     9901 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     9961 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10021 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10081 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10141 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10201 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10261 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    10321 g
//
