LOCUS       Exported               11195 bp DNA     circular SYN 30-DEC-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11195)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11195)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             922..1638
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      1648..2289
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    2516..2540
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3841..4467
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4905..5969
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6038..6232
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6576..6716
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6902..7490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7580..8371)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8796..8820
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8898..9072)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9116..10138)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10205..10881)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11072..11093
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11108..11138
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11146..11162
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11170..11186
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctcg gtacccggga tcctctagag ggcccggcgc gccactagtg
      901 tcgaccagat ctcgtacgcg tatggtgagc aagggcgagg agctgttcac cggggtggtg
      961 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1021 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1081 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1141 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1201 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1261 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1321 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1381 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1441 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1501 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     1561 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1621 atggacgagc tgtacaagtg actgcagagc tttcgttcgt atcatcggtt tcgacaacgt
     1681 tcgtcaagtt caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt
     1741 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     1801 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     1861 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     1921 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     1981 tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac
     2041 cattatgctt attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt
     2101 actgaataca agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt
     2161 ttccagaatc cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt
     2221 tgtagttgag tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac
     2281 atgcatcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2341 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2401 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
     2461 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     2521 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     2581 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     2641 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     2701 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     2761 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     2821 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     2881 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     2941 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     3001 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     3061 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     3121 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     3181 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     3241 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     3301 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     3361 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     3421 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     3481 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     3541 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     3601 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
     3661 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     3721 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     3781 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     3841 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     3901 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     3961 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     4021 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     4081 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     4141 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     4201 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     4261 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     4321 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     4381 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     4441 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     4501 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     4561 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     4621 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     4681 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     4741 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     4801 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
     4861 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     4921 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     4981 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     5041 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     5101 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     5161 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     5221 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     5281 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     5341 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     5401 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     5461 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     5521 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     5581 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     5641 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     5701 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     5761 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     5821 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     5881 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     5941 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     6001 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     6061 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     6121 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     6181 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     6241 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     6301 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     6361 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     6421 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6481 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6541 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     6601 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     6661 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     6721 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6781 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6841 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6901 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6961 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7021 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7081 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7141 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7201 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7261 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7321 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7381 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7441 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7501 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7561 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     7621 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     7681 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     7741 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
     7801 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
     7861 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
     7921 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
     7981 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
     8041 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
     8101 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
     8161 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
     8221 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
     8281 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
     8341 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
     8401 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
     8461 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
     8521 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
     8581 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
     8641 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
     8701 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
     8761 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     8821 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
     8881 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
     8941 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
     9001 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
     9061 attatggaga aactcgagct tgtcgatcga cagatcccgg tcggcatcta ctctatttct
     9121 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
     9181 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
     9241 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
     9301 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg
     9361 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
     9421 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
     9481 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
     9541 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagat
     9601 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
     9661 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
     9721 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat
     9781 ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca
     9841 acgtgacacc ctgtgaacgg cgggagatgc aataggtcag gctctcgcta aactccccaa
     9901 tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat
     9961 ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat
    10021 cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt
    10081 cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat
    10141 ctcattgccc cccggatctg cgaaagctcg agagagatag atttgtagag agagactggt
    10201 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
    10261 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
    10321 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
    10381 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
    10441 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
    10501 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
    10561 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
    10621 ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
    10681 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
    10741 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
    10801 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
    10861 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
    10921 ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca
    10981 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    11041 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    11101 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    11161 aatttcacac aggaaacagc tatgacatga ttacg
//
