LOCUS V022697 5521 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022697 VERSION V022697 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 4..383 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 385..662 /label="chicken β-actin promoter" intron 663..1680 /note="chimera between introns from chicken β-actin and rabbit β-globin" /label="chimeric intron" CDS 1738..1764 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1771..2508 /label="YWHAB(NM_139323)" /note="YWHAB(NM_139323)" /gene="YWHAB" polyA_signal 2608..2663 /note="rabbit β-globin polyadenylation signal (Gil and Proudfoot, 1987)" /label="β-globin poly(A) signal" primer_bind complement(3024..3040) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3048..3064 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3072..3102) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3117..3138 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 3197..3392 /note="SV40 early promoter" /label="SV40 promoter" polyA_signal 3398..3532 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3770..4358) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4529..5389) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5390..5494) /gene="bla" /label="AmpR promoter" ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc 541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt 601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg 661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc 721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg 841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt 901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc 961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg 1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg 1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc 1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc 1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg 1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc 1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg 1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc 1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg 1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt 1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt 1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag 1681 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaagc caccatgtac 1741 ccatacgatg ttccagatta cgctgaattc atgacaatgg ataaaagtga gctggtacag 1801 aaagccaaac tcgctgagca ggctgagcga tatgatgata tggctgcagc catgaaggca 1861 gtcacagaac aggggcatga actctccaac gaagagagaa atctgctctc tgttgcctac 1921 aagaatgtgg taggcgcccg ccgctcttcc tggcgtgtca tctccagcat tgagcagaaa 1981 acagagagga atgagaagaa gcagcagatg ggcaaagagt accgtgagaa gatagaggca 2041 gaactgcagg acatctgcaa tgatgttctg gagctgttgg acaaatatct tattcccaat 2101 gctacacaac cagaaagtaa ggtgttctac ttgaaaatga aaggagatta ttttaggtat 2161 ctttctgaag tggcatctgg agacaacaaa caaaccactg tgtcgaactc ccagcaggct 2221 taccaggaag catttgaaat tagtaagaaa gaaatgcagc ctacacaccc aattcgtctt 2281 ggtctggcac taaatttctc agtcttttac tatgagattc taaactctcc tgaaaaggcc 2341 tgtagcctgg caaaaacggc atttgatgaa gcaattgctg aattggatac gctgaatgaa 2401 gagtcttata aagacagcac tctgatcatg cagttactta gggacaatct cactctgtgg 2461 acatcggaaa accagggaga cgaaggagac gctggggagg gagagaacta actcgagcta 2521 gcagatctta attaattaag gatctttttc cctctgccaa aaattatggg gacatcatga 2581 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt 2641 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac 2701 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga 2761 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct 2821 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat 2881 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc 2941 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc 3001 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 3061 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 3121 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 3181 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 3241 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 3301 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 3361 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa 3421 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 3481 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat 3541 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 3601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 3661 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa 3721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4201 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 4321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4501 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4561 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4621 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4681 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4741 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4801 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 4861 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 4921 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 4981 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5041 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5101 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 5161 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 5221 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 5281 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 5341 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 5401 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 5461 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 5521 g //