LOCUS       V038031                 6631 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038031
VERSION     V038031
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        4..383
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        385..662
                     /label="chicken β-actin promoter"
     intron          663..1680
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
     CDS             1738..1764
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1771..3603
                     /label="KAT7(NM_007067)"
                     /note="KAT7(NM_007067)"
                     /gene="KAT7"
     polyA_signal    3718..3773
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(4134..4150)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4158..4174
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4182..4212)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4227..4248
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        4307..4502
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    4508..4642
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4880..5468)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5639..6499)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6500..6604)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaagc caccatgtac
     1741 ccatacgatg ttccagatta cgctgaattc atgccgcgaa ggaagaggaa tgcaggcagt
     1801 agttcagatg gaaccgaaga ttccgatttt tctacagatc tcgagcacac agacagttca
     1861 gaaagtgatg gcacatcccg acgatctgct cgagtcaccc gctcctcagc caggctaagc
     1921 cagagttctc aagattccag tcctgttcga aatctgcagt cttttggcac tgaggagcct
     1981 gcttactcta ccagaagagt gacccgtagt cagcagcagc ctaccccagt gacaccgaaa
     2041 aaataccctc ttcggcagac tcgttcatct ggttcagaaa ctgagcaagt ggttgatttt
     2101 tcagatagag aaactaaaaa tacagctgat catgatgagt caccgcctcg aactccaact
     2161 ggaaatgcgc cttcttctga gtctgacata gacatctcca gccccaatgt atctcacgat
     2221 gagagcattg ccaaggacat gtccctgaag gactcaggca gtgatctctc tcatcgcccc
     2281 aagcgccgtc gcttccatga aagctacaac ttcaatatga agtgtcctac accaggctgt
     2341 aactctctag gacaccttac aggaaaacat gagagacatt tctccatctc aggatgccca
     2401 ctgtatcata acctctcagc tgacgaatgc aaggtgagag cacagagccg ggataagcag
     2461 atagaagaaa ggatgctgtc tcacaggcaa gatgacaaca acaggcatgc aaccaggcac
     2521 caggcaccaa cggagagaca gcttcgatat aaggaaaaag tggctgaact caggaagaaa
     2581 agaaattctg gactgagcaa agaacagaaa gagaaatata tggaacacag acagacctat
     2641 gggaacacac gggaacctct tttagaaaac ctgacaagcg agtatgactt ggatcttttc
     2701 cgaagagcac aagcccgggc ttcagaggat ttggagaagt taaggctgca aggccaaatc
     2761 acagagggaa gcaacatgat taaaacaatt gcttttggcc gctatgagct tgatacctgg
     2821 tatcattctc catatcctga agaatatgca cggctgggac gtctctatat gtgtgaattc
     2881 tgtttaaaat atatgaagag ccaaacgata ctccgccggc acatggccaa atgtgtgtgg
     2941 aaacacccac ctggtgatga gatatatcgc aaaggttcaa tctctgtgtt tgaagtggat
     3001 ggcaagaaaa acaagatcta ctgccaaaac ctgtgcctgt tggccaaact ttttctggac
     3061 cacaagacat tatattatga tgtggagccc ttcctgttct atgttatgac agaggcggac
     3121 aacactggct gtcacctgat tggatatttt tctaaggaaa agaattcatt cctcaactac
     3181 aacgtctcct gtatccttac tatgcctcag tacatgagac agggctatgg caagatgctt
     3241 attgatttca gttatttgct ttccaaagtc gaagaaaaag ttggctcccc agaacgtcca
     3301 ctctcagatc tggggcttat aagctatcgc agttactgga aagaagtact tctccgctac
     3361 ctgcataatt ttcaaggcaa agagatttct atcaaagaaa tcagtcagga gacggctgtg
     3421 aatcctgtgg acattgtcag cactctgcaa gcccttcaga tgctcaaata ctggaaggga
     3481 aaacacctag ttttaaagag acaggacctg attgatgagt ggatagccaa agaggccaaa
     3541 aggtccaact ccaataaaac catggatccc agctgcttaa aatggacccc tcccaagggc
     3601 acttaaggta cccgggcatg cctcgagcta gcagatctta attaattaag gatctttttc
     3661 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     3721 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     3781 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     3841 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     3901 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     3961 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     4021 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     4081 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
     4141 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4201 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4261 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
     4321 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4381 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4441 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4501 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4561 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4621 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
     4681 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4741 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4801 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4861 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4921 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4981 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5041 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5101 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5161 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5221 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5281 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5341 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5401 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5461 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5521 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5581 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5641 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5701 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5761 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5821 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5881 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5941 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6001 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6061 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6121 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6181 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6241 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6301 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6361 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6421 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6481 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6541 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6601 cgcgcacatt tccccgaaaa gtgccacctg g
//
