LOCUS       pBiT2.1-RPN2(human)     5323 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045683
VERSION     V045683
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     CDS             863..2755
                     /label="RPN2(NM_002951)"
                     /note="RPN2(NM_002951)"
                     /gene="RPN2"
     polyA_signal    2877..2998
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3417..4005)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4205..5065)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    5170..5218
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    5232..5323
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg tggtaaagcc accagatctg ctagcgatcg cctaagtggg
      841 agctcagggg aattccgcca ccatggcgcc gccgggttca agcactgtct tcctgttggc
      901 cctgacaatc atagccagca cctgggctct gacgcccact cactacctca ccaagcatga
      961 cgtggagaga ctaaaagcct cgctggatcg ccctttcaca aatttggaat ctgccttcta
     1021 ctccatcgtg ggactcagca gccttggtgc tcaggtgcca gatgcaaaga aagcatgtac
     1081 ctacatcaga tctaaccttg atcccagcaa tgtggattcc ctcttctacg ctgcccaggc
     1141 cagccaggcc ctctcaggat gtgagatctc tatttcaaat gagaccaaag atctgcttct
     1201 ggcagctgtc agtgaggact catctgttac ccagatctac catgcagttg cagctctaag
     1261 tggctttggc cttcccttgg catcccaaga agcactcagt gcccttactg ctcgtctcag
     1321 caaggaggag actgtgctgg caacagtcca ggctctgcag acagcatccc acctgtccca
     1381 gcaggctgac ctgaggagca tcgtggagga gattgaggac cttgttgctc gcctggatga
     1441 actcgggggc gtgtatctcc agtttgaaga aggactggaa acaacagcgt tatttgtggc
     1501 tgccacctac aagctcatgg atcatgtggg gactgagcca tccattaagg aggatcaggt
     1561 catccagctg atgaacgcga tcttcagcaa gaagaacttt gagtccctct ccgaagcctt
     1621 cagcgtggcc tctgcagctg ctgtgctctc gcataatcgc taccacgtgc cagttgtggt
     1681 tgtgcctgag ggctctgctt ccgacactca tgaacaggct atcttgcggt tgcaagtcac
     1741 caatgttctg tctcagcctc tgactcaggc cactgttaaa ctagaacatg ctaaatctgt
     1801 tgcttccaga gccactgtcc tccagaagac atccttcacc cctgtagggg atgtttttga
     1861 actaaatttc atgaacgtca aattttccag tggttattat gacttccttg tcgaagttga
     1921 aggtgacaac cggtatattg caaataccgt agagctcaga gtcaagatct ccactgaagt
     1981 tggcatcaca aatgttgatc tttccaccgt ggataaggat cagagcattg cacccaaaac
     2041 tacccgggtg acatacccag ccaaagccaa gggcacattc atcgcagaca gccaccagaa
     2101 cttcgccttg ttcttccagc tggtagatgt gaacactggt gctgaactca ctcctcacca
     2161 gacatttgtc cgactccata accagaagac tggccaggaa gtggtgtttg ttgccgagcc
     2221 agacaacaag aacgtgtaca agtttgaact ggatacctct gaaagaaaga ttgaatttga
     2281 ctctgcctct ggcacctaca ctctctactt aatcattgga gatgccactt tgaagaaccc
     2341 aatcctctgg aatgtggctg atgtggtcat caagttccct gaggaagaag ctccctcgac
     2401 tgtcttgtcc cagaaccttt tcactccaaa acaggaaatt cagcacctgt tccgcgagcc
     2461 tgagaagagg ccccccaccg tggtgtccaa tacattcact gccctgatcc tctcgccgtt
     2521 gcttctgctc ttcgctctgt ggatccggat tggtgccaat gtctccaact tcacttttgc
     2581 tcctagcacg attatatttc acctgggaca tgctgctatg ctgggactca tgtatgtcta
     2641 ctggactcag ctcaacatgt tccagacctt gaagtacctg gccatcctgg gcagtgtgac
     2701 gtttctggct ggcaatcgga tgctggccca gcaggcagtc aagagaacag cacattagct
     2761 cgagcggtgg tggcgggagc ggaggtggag ggtcgtcagg tgtgaccggc taccggctgt
     2821 tcgaggagat tctgtaatct agagtcgggg cggccggccg cttcgagcag acatgataag
     2881 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2941 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3001 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     3061 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct
     3121 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     3181 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc
     3241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3841 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4141 atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta aaccactgca
     4201 gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta tttcgttcgt
     4261 ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag
     4321 gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa
     4381 tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg tccgcctcca
     4441 tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc
     4501 gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc ggtatggctt
     4561 cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata ttatgaagaa
     4621 atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc gcggtgttgt
     4681 cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc gtaagatgct
     4741 tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa
     4801 gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt actttgaaag
     4861 tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg ccgctattga
     4921 gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct tttactttca
     4981 ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg
     5041 cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga agcatttatc
     5101 agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg
     5161 acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     5221 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     5281 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//
