LOCUS       V029782                 3705 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029782
VERSION     V029782
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..1219
                     /EC_number=""
                     /codon_start=1
                     /gene=""
                     /gene_synonym=""
                     /note=""
                     /product="human FK506-binding protein FKBP12"
                     /protein_id=""
                     /transl_table=1
                     /translation="M,GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRN
                     KPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVE
                     LLKLE*"
                     /label="FKBP"
     polyA_signal    1259..1380
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(1799..2387)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(2587..3447)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    3552..3600
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    3614..3705
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgggagt
      901 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg
      961 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag
     1021 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg
     1081 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta
     1141 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga
     1201 gcttctaaaa ctggaatgat ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     1261 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1321 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1381 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1441 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     1501 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     1561 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     1621 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1681 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1741 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1801 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1861 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1921 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1981 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2041 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2101 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2161 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2221 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2281 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2341 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2401 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2461 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2521 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg
     2581 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc
     2641 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc
     2701 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc
     2761 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc
     2821 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt
     2881 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc
     2941 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag
     3001 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt
     3061 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg
     3121 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc
     3181 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa
     3241 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt
     3301 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt
     3361 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag
     3421 tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta
     3481 tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt
     3541 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     3601 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
     3661 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct
//
