LOCUS V029782 3705 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029782 VERSION V029782 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter 28..779 /note="herpes simplex virus thymidine kinase promoter" /label="HSV TK promoter" regulatory 809..818 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 893..1219 /EC_number="" /codon_start=1 /gene="" /gene_synonym="" /note="" /product="human FK506-binding protein FKBP12" /protein_id="" /transl_table=1 /translation="M,GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRN KPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVE LLKLE*" /label="FKBP" polyA_signal 1259..1380 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(1799..2387) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(2587..3447) /codon_start=1 /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" polyA_signal 3552..3600 /note="synthetic polyadenylation signal" /label="poly(A) signal" misc_feature 3614..3705 /note="RNA polymerase II transcriptional pause signal from the human α2 globin gene" /label="pause site" ORIGIN 1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa 61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg 121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc 181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg 241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac 301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc 361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc 421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg 481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg 541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg 601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg 661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg 721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa 781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga 841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgggagt 901 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg 961 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag 1021 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg 1081 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta 1141 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga 1201 gcttctaaaa ctggaatgat ctagagtcgg ggcggccggc cgcttcgagc agacatgata 1261 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 1321 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 1381 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 1441 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc 1501 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc 1561 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt 1621 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 1681 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 1741 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 1801 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 1861 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 1921 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 1981 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 2041 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 2101 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 2161 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 2221 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 2281 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 2341 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 2401 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 2461 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 2521 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg 2581 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc 2641 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc 2701 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc 2761 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc 2821 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt 2881 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc 2941 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag 3001 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt 3061 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg 3121 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc 3181 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa 3241 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt 3301 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt 3361 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag 3421 tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta 3481 tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt 3541 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg 3601 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa 3661 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct //