LOCUS       62056_4320        4514 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..33
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     sig_peptide     79..141
                     /label=OmpA signal peptide
                     /note="signal peptide from the E. coli outer membrane
                     protein OmpA"
     CDS             838..855
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1087..1173
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1265..1292
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1311..1402
                     /label=AmpR promoter
     CDS             1403..2260
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2434..3022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3377..4087)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 gaattcatgt ttcacacagg aaacagctat gaccatgatt acggattcac tggaactcta
       61 gataacgagg cgcaaaaaat gaaaaagaca gctatcgcga ttgcagtggc actggcaggt
      121 ttcgctaccg tcgctcaggc tatgaaatgg gttaccttca tctctctgct gttcctgttc
      181 tcttctgctt actctggttc ttggctgctg gaagttccga acggtccgtg gcgttctctg
      241 accttctacc cggcttggct gaccgtttct gaaggtgcta acgctacctt cacctgctct
      301 ctgtctaact ggtctgaaga cctgatgctg aactggaacc gtctgtctcc gtctaaccag
      361 accgaaaaac aggctgcttt ctgcaacggt ctgtctcagc cggttcagga cgctcgtttc
      421 cagatcatcc agctgccgaa ccgtcacgac ttccacatga acatcctgga cacccgtcgt
      481 aacgactctg gtatctacct gtgcggtgct atctctctgc acccgaaagc taaaatcgaa
      541 gaatctccgg gtgctgaact ggttgttacc gaacgtatcc tggaaggtcc gcagccgcag
      601 ccgaaaccgc agccgaaacc ggaaccggaa ccgcagccgc agggtggttc tgaagaagac
      661 ccgtgcgctt gcgaatctat cctgaaattc gaagctaaag ttgaaggtct gctgcaggct
      721 ctgacccgta aactggaagc tgtttctggt cgtctggctg ttctggaaaa ccgtatcatc
      781 gctgctgctg gtgctccggt tccgtacccg gacccgctgg aaccgcgtgg tggttctcac
      841 caccaccacc accactaagg atccgtcgac ctgcagccaa gcttggctgt tttggcggat
      901 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
      961 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     1021 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     1081 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1141 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     1201 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
     1261 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt
     1321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1381 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1441 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1501 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1561 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1621 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     1681 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1741 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1801 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1861 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1921 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1981 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2041 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2101 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2161 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2221 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2281 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2341 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2581 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3061 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3121 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3181 gtcagtgagc gaggaagcgg aagagcgctt atctttccct ttatttttgc tgcggtaagt
     3241 cgcataaaaa ccattcttca taattcaatc catttactat gttatgttct gaggggagtg
     3301 aaaattcccc taattcgatg aagattcttg ctcaattgtt atcagctatg cgccgaccag
     3361 aacaccttgc cgatcagcca aacgtctctt caggccactg actagcgata actttcccca
     3421 caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt ggttgtaaaa
     3481 acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg
     3541 ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt aacattccgt
     3601 caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca acctcaagcc
     3661 agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca tcacctttgg
     3721 taaaggttct aagcttaggt gagaacatcc ctgcctgaac atgagaaaaa acagggtact
     3781 catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg tagatttctc
     3841 tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgta agcaatgcgg
     3901 cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct gactgcccca
     3961 tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc tttttttcat
     4021 aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg
     4081 tgctcatacg ttaaatctat caccgcaagg gataaatatc taacaccgtg cgtgttgact
     4141 attttacctc tggcggtgat aatggttgca tgtactaagg aggttgtatg gaacaacgca
     4201 taaccctgaa agattatgca atgcgctttg ggcaaaccaa gacagctaaa gatctctcac
     4261 ctaccaaaca atgcccccct gcaaaaaata aattcatata aaaaacatac agataaccat
     4321 ctgcggtgat aaattatctc tggcggtgtt gacataaata ccactggcgg tgatactgag
     4381 cacatcagca ggacgcactg accaccatga aggtgacgct cttaaaaatt aagccctgaa
     4441 gaagggcagc attcaaagca gaaggctttg gggtgtgtga tacgaaacga agcattggtt
     4501 aaaaattaag gagg
//
