LOCUS       62056_3785        8900 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        801..905
                     /label=AmpR promoter
     CDS             906..1763
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1937..2525
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2964..3986
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6050..7051)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             8146..8862
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8869..8886
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
ORIGIN
        1 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
       61 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
      121 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
      181 ggtaacgccg ttccgcaccc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
      241 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      301 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      361 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      421 catctccttg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      481 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgat atggtgcact ctcagtacaa
      541 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
      601 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
      661 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
      721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1141 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1441 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2581 acatgttact acctgtccct tgctgatttt taaacgagca cgagagcaaa accccccttt
     2641 gctgaggtgg cagagggcag gtttttttgt ttcttttttc tcgtaaaaaa aagaaaggtc
     2701 ttaaaggttt tatggttttg gtcggcactg ccgacagcct cgcagagcac acactttatg
     2761 aatataaagt atagtgtgtt atactttact tggaagtggt tgccggaaag agcgaaaatg
     2821 cctcacattt gtgccaccta aaaaggagcg atttacatat gtttcaaatg gccaaacgcg
     2881 ttctcctcag taccacgcta acgttcagcc ttcttgccgg cagtgcattg cctttcctgc
     2941 ctgcttccgc gatttatgcc gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     3001 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     3061 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     3121 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     3181 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     3241 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     3301 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     3361 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     3421 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     3481 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     3541 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     3601 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     3661 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     3721 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     3781 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     3841 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     3901 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     3961 ccagcactcg tccgagggca aaggaatagt atgtaaatcg ctccttttta ggtggcacaa
     4021 atgtgaggca ttttcgctct ttccggcaac cacttccaag taaagtataa cacactatac
     4081 tttatattca taaagtgtgt gctctgcgag gctgtcggca gtgccgacca aaaccataaa
     4141 acctttaaga cctttctttt ttttacgaga aaaaagaaac aaaaaaacct gccctctgcc
     4201 acctcagcaa aggggggttt tgctctcgtg ctcgtttaaa aatcagcaag ggacaggtag
     4261 tattttttga gaagatcact caaaaaatct ccacctttaa acccttgcca atttttattt
     4321 tgtccgtttt gtctagctta ccgaaagcca gactcagcaa gaataaaatt tttattgtct
     4381 ttcggttttc tagtgtaacg gacaaaacca ctcaaaataa aaaagataca agagaggtct
     4441 ctcgtatctt ttattcagca atcgcgcccg attgctgaac agattaataa tagattttag
     4501 ctttttattt gttgaaaaaa gctaatcaaa ttgttgtcgg gatcaattac tgcaaagtct
     4561 cgttcatccc accactgatc ttttaatgat gtattggggt gcaaaatgcc caaaggctta
     4621 atatgttgat ataattcatc aattccctct acttcaatgc ggcaactagc agtaccagca
     4681 ataaacgact ccgcacctgt acaaaccggt gaatcattac tacgagagcg ccagcttcat
     4741 cacttgcctc ccatagatga atccgaacct cattacacat tagaactgcg aatccatctt
     4801 catggtgaac caaagtgaaa cctagtttat cgcaataaaa acctatactc tttttaatat
     4861 ccccgactgg caatgccggg atagactgta acattctcac gcataaaatc ccctttcatt
     4921 ttctaatgta aatctattac cttattatta attcaattcg ctcataatta atcctttttc
     4981 ttattacgca aaatggcccg atttaagcac accctttatt ccgttaatgc gccatgacag
     5041 ccatgataat tactaatact aggagaagtt aataaatacg taaccaacat gattaacaat
     5101 tattagaggt catcgttcaa aatggtatgc gttttgacac atccactata tatccgtgtc
     5161 gttctgtcca ctcctgaatc ccattccaga aattctctag cgattccaga agtttctcag
     5221 agtcggaaag ttgaccagac attacgaact ggcacagatg gtcataacct gaaggaagat
     5281 ctgattgctt aactgcttca gttaagaccg aagcgctcgt cgtataacag atgcgatgat
     5341 gcagaccaat caacatggca cctgccattg ctacctgtac agtcaaggat ggtagaaatg
     5401 ttgtcggtcc ttgcacacga atattacgcc atttgcctgc atattcaaac agctcttcta
     5461 cgataagggc acaaatcgca tcgtggaacg tttgggcttc taccgattta gcagtttgat
     5521 acactttctc taagtatcca cctgaatcat aaatcggcaa aatagagaaa aattgaccat
     5581 gtgtaagcgg ccaatctgat tccacctgag atgcataatc tagtagaatc tcttcgctat
     5641 caaaattcac ttccaccttc cactcaccgg ttgtccattc atggctgaac tctgcttcct
     5701 ctgttgacat gacacacatc atctcaatat ccgaataggg cccatcagtc tgacgaccaa
     5761 gagagccata aacaccaata gccttaacat catccccata tttatccaat attcgttcct
     5821 taatttcatg aacaatcttc attctttctt ctctagtcat tattattggt ccattcacta
     5881 ttctcattcc cttttcagat aattttagat ttgcttttct aaataagaat atttggagag
     5941 caccgttctt attcagctat taataactcg tcttcctaag catccttcaa tccttttaat
     6001 aacaattata gcatctaatc ttcaacaaac tggcccgttt gttgaactac tctttaataa
     6061 aataattttt ccgttcccaa ttccacattg caataataga aaatccatct tcatcggctt
     6121 tttcgtcatc atctgtatga atcaaatcgc cttcttctgt gtcatcaagg tttaattttt
     6181 tatgtatttc ttttaacaaa ccaccatagg agattaacct tttacggtgt aaaccttcct
     6241 ccaaatcaga caaacgtttc aaattctttt cttcatcatc ggtcataaaa tccgtatcct
     6301 ttacaggata ttttgcagtt tcgtcaattg ccgattgtat atccgattta tatttatttt
     6361 tcggtcgaat catttgaact tttacatttg gatcatagtc taatttcatt gcctttttcc
     6421 aaaattgaat ccattgtttt tgattcacgt agttttctgt attcttaaaa taagttggtt
     6481 ccacacatac caatacatgc atgtgctgat tataagaatt atctttatta tttattgtca
     6541 cttccgttgc acgcataaaa ccaacaagat ttttattaat ttttttatat tgcatcattc
     6601 ggcgaaatcc ttgagccata tctgacaaac tcttatttaa ttcttcgcca tcataaacat
     6661 ttttaactgt taatgtgaga aacaaccaac gaactgttgg cttttgttta ataacttcag
     6721 caacaacctt ttgtgactga atgccatgtt tcattgctct cctccagttg cacattggac
     6781 aaagcctgga tttacaaaac cacactcgat acaactttct ttcgcctgtt tcacgatttt
     6841 gtttatactc taatatttca gcacaatctt ttactctttc agccttttta aattcaagaa
     6901 tatgcagaag ttcaaagtaa tcaacattag cgattttctt ttctctccat ggtctcactt
     6961 ttccactttt tgtcttgtcc actaaaaccc ttgatttttc atctgaataa atgctactat
     7021 taggacacat aatattaaaa gaaaccccca tctatttagt tatttgttta gtcacttata
     7081 actttaacag atggggtttt tctgtgcaac caattttaag ggttttcaat actttaaaac
     7141 acatacatac caacacttca acgcaccttt cagcaactaa aataaaaatg acgttatttc
     7201 tatatgtatc aagataagaa agaacaagtt caaaaccatc aaaaaaagac accttttcag
     7261 gtgctttttt tattttataa actcattccc tgatctcgac ttcgttcttt ttttacctct
     7321 cggttatgag ttagttcaaa ttcgttcttt ttaggttcta aatcgtgttt ttcttggaat
     7381 tgtgctgttt tatcctttac cttgtctaca aaccccttaa aaacgttttt aaaggctttt
     7441 aagccgtctg tacgttcctt aaggaattcg agctcagctt tattgagtgg atgattatat
     7501 tccttttgat aggtggtatg ttttcgcttg aacttttaaa tacagccatt gaacatacgg
     7561 ttgatttaat aactgacaaa catcaccctc ttgctaaagc ggccaaggac gctgccgccg
     7621 gggctgtttg cgtttttgcc gtgatttcgt gtattattgg tttacttatt tttttgccaa
     7681 agctgtaatg gctgaaaatt cttacattta ttttacattt ttagaaatgg gcgtgaaaaa
     7741 aagcgcgcga ttatgtaaaa tataaagtga tagcggtacc attataggta agagaggaat
     7801 gtacacatga agaaaccgtt ggggaaaatt gtcgcaagca ccgcactact catttctgtt
     7861 gcttttagtt catcgatcgc atcggctgct gaagaagcaa aagaaaaata tttaattggc
     7921 tttaatgagc aggaagctgt cagtgagttt gtagaacaag tagaggcaaa tgacgaggtc
     7981 gccattctct ctgaggaaga ggaagtcgaa attgaattgc ttcatgaatt tgaaacgatt
     8041 cctgttttat ccgttgagtt aagcccagaa gatgtggacg cgcttgaact cgatccagcg
     8101 atttcttata ttgaagagga tgcagaagta acgacaatgg gatccatggt gagcaagggc
     8161 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     8221 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     8281 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     8341 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     8401 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     8461 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     8521 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     8581 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     8641 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     8701 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     8761 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     8821 accgccgccg ggatcactct cggcatggac gagctgtaca agctcgagca ccaccaccac
     8881 caccacaagc ttgtattcta
//
