LOCUS       62056_3770        7709 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1095..1811
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1818..1835
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        complement(1846..1864)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        2648..2752
                     /label=AmpR promoter
     CDS             2753..3610
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3784..4372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6708..7709)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
ORIGIN
        1 ctatttagtt atttgtttag tcacttataa ctttaacaga tggggttttt ctgtgcaacc
       61 aattttaagg gttttcaata ctttaaaaca catacatacc aacacttcaa cgcacctttc
      121 agcaactaaa ataaaaatga cgttatttct atatgtatca agataagaaa gaacaagttc
      181 aaaaccatca aaaaaagaca ccttttcagg tgcttttttt attttataaa ctcattccct
      241 gatctcgact tcgttctttt tttacctctc ggttatgagt tagttcaaat tcgttctttt
      301 taggttctaa atcgtgtttt tcttggaatt gtgctgtttt atcctttacc ttgtctacaa
      361 accccttaaa aacgttttta aaggctttta agccgtctgt acgttcctta aggaattcga
      421 gctcagcttt attgagtgga tgattatatt ccttttgata ggtggtatgt tttcgcttga
      481 acttttaaat acagccattg aacatacggt tgatttaata actgacaaac atcaccctct
      541 tgctaaagcg gccaaggacg ctgccgccgg ggctgtttgc gtttttgccg tgatttcgtg
      601 tattattggt ttacttattt ttttgccaaa gctgtaatgg ctgaaaattc ttacatttat
      661 tttacatttt tagaaatggg cgtgaaaaaa agcgcgcgat tatgtaaaat ataaagtgat
      721 agcggtacca ttataggtaa gagaggaatg tacacatgaa gaaaccgttg gggaaaattg
      781 tcgcaagcac cgcactactc atttctgttg cttttagttc atcgatcgca tcggctgctg
      841 aagaagcaaa agaaaaatat ttaattggct ttaatgagca ggaagctgtc agtgagtttg
      901 tagaacaagt agaggcaaat gacgaggtcg ccattctctc tgaggaagag gaagtcgaaa
      961 ttgaattgct tcatgaattt gaaacgattc ctgttttatc cgttgagtta agcccagaag
     1021 atgtggacgc gcttgaactc gatccagcga tttcttatat tgaagaggat gcagaagtaa
     1081 cgacaatggg atccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1141 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1201 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1261 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1321 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1381 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1441 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1501 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1561 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     1621 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1681 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     1741 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1801 agctgtacaa gctcgagcac caccaccacc accacaagct tgtattctat agtgtcacct
     1861 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
     1921 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
     1981 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
     2041 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
     2101 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     2161 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     2221 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     2281 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     2341 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     2401 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2461 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2521 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     2581 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     2641 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2701 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2761 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2821 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2881 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2941 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3001 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3061 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3121 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3181 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3241 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3301 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3361 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3421 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3481 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3541 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3601 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3661 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3721 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3781 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3841 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3901 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3961 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4021 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4081 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4141 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4201 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4261 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4321 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4381 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4441 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4501 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4561 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gatttcactt
     4621 tttgcattct acaaactgca taactcatat gtaaatcgct cctttttagg tggcacaaat
     4681 gtgaggcatt ttcgctcttt ccggcaacca cttccaagta aagtataaca cactatactt
     4741 tatattcata aagtgtgtgc tctgcgaggc tgtcggcagt gccgaccaaa accataaaac
     4801 ctttaagacc tttctttttt ttacgagaaa aaagaaacaa aaaaacctgc cctctgccac
     4861 ctcagcaaag gggggttttg ctctcgtgct cgtttaaaaa tcagcaaggg acaggtagta
     4921 ttttttgaga agatcactca aaaaatctcc acctttaaac ccttgccaat ttttattttg
     4981 tccgttttgt ctagcttacc gaaagccaga ctcagcaaga ataaaatttt tattgtcttt
     5041 cggttttcta gtgtaacgga caaaaccact caaaataaaa aagatacaag agaggtctct
     5101 cgtatctttt attcagcaat cgcgcccgat tgctgaacag attaataata gattttagct
     5161 ttttatttgt tgaaaaaagc taatcaaatt gttgtcggga tcaattactg caaagtctcg
     5221 ttcatcccac cactgatctt ttaatgatgt attggggtgc aaaatgccca aaggcttaat
     5281 atgttgatat aattcatcaa ttccctctac ttcaatgcgg caactagcag taccagcaat
     5341 aaacgactcc gcacctgtac aaaccggtga atcattacta cgagagcgcc agcttcatca
     5401 cttgcctccc atagatgaat ccgaacctca ttacacatta gaactgcgaa tccatcttca
     5461 tggtgaacca aagtgaaacc tagtttatcg caataaaaac ctatactctt tttaatatcc
     5521 ccgactggca atgccgggat agactgtaac attctcacgc ataaaatccc ctttcatttt
     5581 ctaatgtaaa tctattacct tattattaat tcaattcgct cataattaat cctttttctt
     5641 attacgcaaa atggcccgat ttaagcacac cctttattcc gttaatgcgc catgacagcc
     5701 atgataatta ctaatactag gagaagttaa taaatacgta accaacatga ttaacaatta
     5761 ttagaggtca tcgttcaaaa tggtatgcgt tttgacacat ccactatata tccgtgtcgt
     5821 tctgtccact cctgaatccc attccagaaa ttctctagcg attccagaag tttctcagag
     5881 tcggaaagtt gaccagacat tacgaactgg cacagatggt cataacctga aggaagatct
     5941 gattgcttaa ctgcttcagt taagaccgaa gcgctcgtcg tataacagat gcgatgatgc
     6001 agaccaatca acatggcacc tgccattgct acctgtacag tcaaggatgg tagaaatgtt
     6061 gtcggtcctt gcacacgaat attacgccat ttgcctgcat attcaaacag ctcttctacg
     6121 ataagggcac aaatcgcatc gtggaacgtt tgggcttcta ccgatttagc agtttgatac
     6181 actttctcta agtatccacc tgaatcataa atcggcaaaa tagagaaaaa ttgaccatgt
     6241 gtaagcggcc aatctgattc cacctgagat gcataatcta gtagaatctc ttcgctatca
     6301 aaattcactt ccaccttcca ctcaccggtt gtccattcat ggctgaactc tgcttcctct
     6361 gttgacatga cacacatcat ctcaatatcc gaatagggcc catcagtctg acgaccaaga
     6421 gagccataaa caccaatagc cttaacatca tccccatatt tatccaatat tcgttcctta
     6481 atttcatgaa caatcttcat tctttcttct ctagtcatta ttattggtcc attcactatt
     6541 ctcattccct tttcagataa ttttagattt gcttttctaa ataagaatat ttggagagca
     6601 ccgttcttat tcagctatta ataactcgtc ttcctaagca tccttcaatc cttttaataa
     6661 caattatagc atctaatctt caacaaactg gcccgtttgt tgaactactc tttaataaaa
     6721 taatttttcc gttcccaatt ccacattgca ataatagaaa atccatcttc atcggctttt
     6781 tcgtcatcat ctgtatgaat caaatcgcct tcttctgtgt catcaaggtt taatttttta
     6841 tgtatttctt ttaacaaacc accataggag attaaccttt tacggtgtaa accttcctcc
     6901 aaatcagaca aacgtttcaa attcttttct tcatcatcgg tcataaaatc cgtatccttt
     6961 acaggatatt ttgcagtttc gtcaattgcc gattgtatat ccgatttata tttatttttc
     7021 ggtcgaatca tttgaacttt tacatttgga tcatagtcta atttcattgc ctttttccaa
     7081 aattgaatcc attgtttttg attcacgtag ttttctgtat tcttaaaata agttggttcc
     7141 acacatacca atacatgcat gtgctgatta taagaattat ctttattatt tattgtcact
     7201 tccgttgcac gcataaaacc aacaagattt ttattaattt ttttatattg catcattcgg
     7261 cgaaatcctt gagccatatc tgacaaactc ttatttaatt cttcgccatc ataaacattt
     7321 ttaactgtta atgtgagaaa caaccaacga actgttggct tttgtttaat aacttcagca
     7381 acaacctttt gtgactgaat gccatgtttc attgctctcc tccagttgca cattggacaa
     7441 agcctggatt tacaaaacca cactcgatac aactttcttt cgcctgtttc acgattttgt
     7501 ttatactcta atatttcagc acaatctttt actctttcag cctttttaaa ttcaagaata
     7561 tgcagaagtt caaagtaatc aacattagcg attttctttt ctctccatgg tctcactttt
     7621 ccactttttg tcttgtccac taaaaccctt gatttttcat ctgaataaat gctactatta
     7681 ggacacataa tattaaaaga aacccccat
//
