LOCUS       V015254                 7700 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015254
VERSION     V015254
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      891..1479
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3531..4178)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(4504..5505)
                     /label="repB"
                     /note="RepB replication protein"
     CDS             6624..6641
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        complement(6652..6670)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        7455..7559
                     /label="AmpR promoter"
     CDS             join(7560..7700,1..717)
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
       61 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      121 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      181 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      241 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      301 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
      361 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
      421 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
      481 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
      541 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
      601 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
      661 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
      721 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
      781 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      841 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      901 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      961 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1021 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     1081 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1141 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1201 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1261 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1321 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1381 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1441 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1501 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1561 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1621 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     1681 cctctccccg cgcgttggcc gattcattaa tgcagctgat ttcacttttt gcattctaca
     1741 aactgcataa ctcatatgta aatcgctcct ttttaggtgg cacaaatgtg aggcattttc
     1801 gctctttccg gcaaccactt ccaagtaaag tataacacac tatactttat attcataaag
     1861 tgtgtgctct gcgaggctgt cggcagtgcc gaccaaaacc ataaaacctt taagaccttt
     1921 ctttttttta cgagaaaaaa gaaacaaaaa aacctgccct ctgccacctc agcaaagggg
     1981 ggttttgctc tcgtgctcgt ttaaaaatca gcaagggaca ggtagtattt tttgagaaga
     2041 tcactcaaaa aatctccacc tttaaaccct tgccaatttt tattttgtcc gttttgtcta
     2101 gcttaccgaa agccagactc agcaagaata aaatttttat tgtctttcgg ttttctagtg
     2161 taacggacaa aaccactcaa aataaaaaag atacaagaga ggtctctcgt atcttttatt
     2221 cagcaatcgc gcccgattgc tgaacagatt aataatagat tttagctttt tatttgttga
     2281 aaaaagctaa tcaaattgtt gtcgggatca attactgcaa agtctcgttc atcccaccac
     2341 tgatctttta atgatgtatt ggggtgcaaa atgcccaaag gcttaatatg ttgatataat
     2401 tcatcaattc cctctacttc aatgcggcaa ctagcagtac cagcaataaa cgactccgca
     2461 cctgtacaaa ccggtgaatc attactacga gagcgccagc ttcatcactt gcctcccata
     2521 gatgaatccg aacctcatta cacattagaa ctgcgaatcc atcttcatgg tgaaccaaag
     2581 tgaaacctag tttatcgcaa taaaaaccta tactcttttt aatatccccg actggcaatg
     2641 ccgggataga ctgtaacatt ctcacgcata aaatcccctt tcattttcta atgtaaatct
     2701 attaccttat tattaattca attcgctcat aattaatcct ttttcttatt acgcaaaatg
     2761 gcccgattta agcacaccct ttattccgtt aatgcgccat gacagccatg ataattacta
     2821 atactaggag aagttaataa atacgtaacc aacatgatta acaattatta gaggtcatcg
     2881 ttcaaaatgg tatgcgtttt gacacatcca ctatatatcc gtgtcgttct gtccactcct
     2941 gaatcccatt ccagaaattc tctagcgatt ccagaagttt ctcagagtcg gaaagttgac
     3001 cagacattac gaactggcac agatggtcat aacctgaagg aagatctgat tgcttaactg
     3061 cttcagttaa gaccgaagcg ctcgtcgtat aacagatgcg atgatgcaga ccaatcaaca
     3121 tggcacctgc cattgctacc tgtacagtca aggatggtag aaatgttgtc ggtccttgca
     3181 cacgaatatt acgccatttg cctgcatatt caaacagctc ttctacgata agggcacaaa
     3241 tcgcatcgtg gaacgtttgg gcttctaccg atttagcagt ttgatacact ttctctaagt
     3301 atccacctga atcataaatc ggcaaaatag agaaaaattg accatgtgta agcggccaat
     3361 ctgattccac ctgagatgca taatctagta gaatctcttc gctatcaaaa ttcacttcca
     3421 ccttccactc accggttgtc cattcatggc tgaactctgc ttcctctgtt gacatgacac
     3481 acatcatctc aatatccgaa tagggccaca gtcggcatta tctcatatta taaaagccag
     3541 tcattaggcc tatctgacaa ttcctgaata gagttcataa acaatcctgc atgataacca
     3601 tcacaaacag aatgatgtac ctgtaaagat agcggtaaat atattgaatt acctttatta
     3661 atgaattttc ctgctgtaat aatgggtaga aggtaattac tattattatt gatatttaag
     3721 ttaaacccag taaatgaagt ccatggaata atagaaagag aaaaagcatt ttcaggtata
     3781 ggtgttttgg gaaacaattt ccccgaacca ttatatttct ctacatcaga aaggtataaa
     3841 tcataaaact ctttgaagtc attctttaca ggagtccaaa taccagagaa tgttttagat
     3901 acaccatcaa aaattgtata aagtggctct aacttatccc aataacctaa ctctccgtcg
     3961 ctattgtaac cagttctaaa agctgtattt gagtttatca cccttgtcac taagaaaata
     4021 aatgcagggt aaaatttata tccttcttgt tttatgtttc ggtataaaac actaatatca
     4081 atttctgtgg ttatactaaa agtcgtttgt tggttcaaat aatgattaaa tatctctttt
     4141 ctcttccaat tgtctaaatc aattttatta aagttcattt gatatgcctc ctggcccatc
     4201 agtctgacga ccaagagagc cataaacacc aatagcctta acatcatccc catatttatc
     4261 caatattcgt tccttaattt catgaacaat cttcattctt tcttctctag tcattattat
     4321 tggtccattc actattctca ttcccttttc agataatttt agatttgctt ttctaaataa
     4381 gaatatttgg agagcaccgt tcttattcag ctattaataa ctcgtcttcc taagcatcct
     4441 tcaatccttt taataacaat tatagcatct aatcttcaac aaactggccc gtttgttgaa
     4501 ctactcttta ataaaataat ttttccgttc ccaattccac attgcaataa tagaaaatcc
     4561 atcttcatcg gctttttcgt catcatctgt atgaatcaaa tcgccttctt ctgtgtcatc
     4621 aaggtttaat tttttatgta tttcttttaa caaaccacca taggagatta accttttacg
     4681 gtgtaaacct tcctccaaat cagacaaacg tttcaaattc ttttcttcat catcggtcat
     4741 aaaatccgta tcctttacag gatattttgc agtttcgtca attgccgatt gtatatccga
     4801 tttatattta tttttcggtc gaatcatttg aacttttaca tttggatcat agtctaattt
     4861 cattgccttt ttccaaaatt gaatccattg tttttgattc acgtagtttt ctgtattctt
     4921 aaaataagtt ggttccacac ataccaatac atgcatgtgc tgattataag aattatcttt
     4981 attatttatt gtcacttccg ttgcacgcat aaaaccaaca agatttttat taattttttt
     5041 atattgcatc attcggcgaa atccttgagc catatctgac aaactcttat ttaattcttc
     5101 gccatcataa acatttttaa ctgttaatgt gagaaacaac caacgaactg ttggcttttg
     5161 tttaataact tcagcaacaa ccttttgtga ctgaatgcca tgtttcattg ctctcctcca
     5221 gttgcacatt ggacaaagcc tggatttaca aaaccacact cgatacaact ttctttcgcc
     5281 tgtttcacga ttttgtttat actctaatat ttcagcacaa tcttttactc tttcagcctt
     5341 tttaaattca agaatatgca gaagttcaaa gtaatcaaca ttagcgattt tcttttctct
     5401 ccatggtctc acttttccac tttttgtctt gtccactaaa acccttgatt tttcatctga
     5461 ataaatgcta ctattaggac acataatatt aaaagaaacc cccatctatt tagttatttg
     5521 tttagtcact tataacttta acagatgggg tttttctgtg caaccaattt taagggtttt
     5581 caatacttta aaacacatac ataccaacac ttcaacgcac ctttcagcaa ctaaaataaa
     5641 aatgacgtta tttctatatg tatcaagata agaaagaaca agttcaaaac catcaaaaaa
     5701 agacaccttt tcaggtgctt tttttatttt ataaactcat tccctgatct cgacttcgtt
     5761 ctttttttac ctctcggtta tgagttagtt caaattcgtt ctttttaggt tctaaatcgt
     5821 gtttttcttg gaattgtgct gttttatcct ttaccttgtc tacaaacccc ttaaaaacgt
     5881 ttttaaaggc ttttaagccg tctgtacgtt ccttaaggaa ttcgagctca gcattattga
     5941 gtggatgatt atattccttt tgataggtgg tatgttttcg cttgaacttt taaatacagc
     6001 cattgaacat acggttgatt taataactga caaacatcac cctcttgcta aagcggccaa
     6061 ggacgctgcc gccggggctg tttgcgtttt tgccgtgatt tcgtgtatta ttggtttact
     6121 tatttttttg ccaaagctgt aatggctgaa aattcttaca tttattttac atttttagaa
     6181 atgggcgtga aaaaaagcgc gcgattatgt aaaatataaa gtgatagcgg taccattata
     6241 ggtaagagag gaatgtacac atgaagaaac cgttggggaa aattgtcgca agcaccgcac
     6301 tactcatttc tgttgctttt agttcatcga tcgcatcggc tgctgaagaa gcaaaagaaa
     6361 aatatttaat tggctttaat gagcaggaag ctgtcagtga gtttgtagaa caagtagagg
     6421 caaatgacga ggtcgccatt ctctctgagg aagaggaagt cgaaattgaa ttgcttcatg
     6481 aatttgaaac gattcctgtt ttatccgttg agttaagccc agaagatgtg gacgcgcttg
     6541 aactcgatcc agcgatttct tatattgaag aggatgcaga agtaacgaca atgggatcct
     6601 ctagagtcga cctgcagctc gagcaccacc accaccacca caagcttgta ttctatagtg
     6661 tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa
     6721 cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc
     6781 tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa
     6841 cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat
     6901 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     6961 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     7021 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     7081 cttgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     7141 ctaatgcagg agtcgcataa gggagagcgt cgatatggtg cactctcagt acaatctgct
     7201 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7261 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7321 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7381 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7441 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7501 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7561 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7621 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7681 gagtgggtta catcgaactg
//
