LOCUS       pAbAi.        4894 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast integrating vector for cloning a DNA bait sequence to be used 
            in a one-hybrid screen.
ACCESSION   .
VERSION     .
KEYWORDS    pAbAi.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize with BstBI or BbsI to integrate at the URA3 locus.
FEATURES             Location/Qualifiers
     source          1..4894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..45
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             185..1387
                     /label=AurR
                     /note="aureobasidin A-resistant mutant of S. cerevisiae
                     inositol phosphorylceramide synthase (Aeed et al., 2009)"
     primer_bind     complement(1645..1661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1669..1685)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1693..1723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1738..1759)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2047..2635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2809..3666)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3667..3771)
                     /label=AmpR promoter
     CDS             complement(3878..4678)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4679..4894)
                     /label=URA3 promoter
ORIGIN
        1 aagcttgaat tcgagctcgg tacccgggga tctgtcgacc tcgaggcatg tgctctgtat
       61 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc
      121 tttgtagcat aaattactat acttctatag acacgcaaac acaaatacac acactaaatt
      181 aataatggca aacccttttt cgagatggtt tctatcagag agacctccaa actgccatgt
      241 agccgattta gaaacaagtt tagatcccca tcaaacgttg ttgaaggtgc aaaaatacaa
      301 acccgcttta agcgactggg tgcattacat cttcttggga tccatcatgc tgtttgtgtt
      361 cattactaat cccgcacctt ggatcttcaa gatccttttt tattgtttct tgggcacttt
      421 attcatcatt ccagctacgt cacagttttt cttcaatgcc ttgcccatcc taacatgggt
      481 ggcgctgtat ttcacttcat cgtactttcc agatgaccgc aggcctccta ttactgtcaa
      541 agtgttacca gcggtggaaa caattttata cggcgacaat ttaagtgata ttcttgcaac
      601 atcgacgaat tcctttttgg acattttagc atggttaccg tacggactat ttcattatgg
      661 ggccccattt gtcgttgctg ccatcttatt cgtatttggt ccaccaactg ttttgcaagg
      721 ttatgctttt gcatttggtt atatgaacct gtttggtgtt atcatgcaaa atgtctttcc
      781 agccgctccc ccatggtata aaattctcta tggattgcaa tcagccaact atgatatgca
      841 tggctcgcct ggtggattag ctagaattga taagctactc ggtattaata tgtatactac
      901 atgtttttca aattcctccg tcattttcgg tgcttttcct tcactgcatt ccgggtgtgc
      961 tactatggaa gccctgtttt tctgttattg ttttccaaaa ttgaagccct tgtttattgc
     1021 ttatgtttgc tggttatggt ggtcaactat gtatctgaca caccattatt ttgtagacct
     1081 tatggcaggt tctgtgctgt catacgttat tttccagtac acaaagtaca cacatttacc
     1141 aattgtagat acatctcttt tttgcagatg gtcatacact tcaattgaga aatacgatat
     1201 atcaaagagt gatccattgg ctgcagattc aaacgatatc gaaagtgtcc ctttgtccaa
     1261 cttggaactt gactttgatc ttaatatgac tgatgaaccc agtgtaagcc cttcgttatt
     1321 tgatggatct acttctgttt ctcgttcgtc cgccacgtct ataacgtcac taggtgtaaa
     1381 gagggcttaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt
     1441 ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg
     1501 ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct
     1561 ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact cggcctcgac
     1621 tctagcgaat taattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     1681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3421 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3781 ccgaaaagtg ccacctgggt aataactgat ataattaaat tgaagctcta atttgtgagt
     3841 ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca
     3901 aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct
     3961 ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca
     4021 cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca
     4081 taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga
     4141 taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag
     4201 atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta
     4261 cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat
     4321 tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg
     4381 tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata
     4441 atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg
     4501 tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg
     4561 tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct
     4621 tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga
     4681 tttatcttcg tttcctgcag gtttttgttc tgtgcagttg ggttaagaat actgggcaat
     4741 ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc
     4801 ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga aaccgaaatc
     4861 aaaaaaaaga ataaaaaaaa aatgatgaat tgaa
//
