LOCUS       62056_3105        6054 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     309..325
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        341..2474
                     /label=gpdA promoter
                     /note="promoter from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     intron          2523..2638
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             2647..3018
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      3026..3801
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene (Punt et al., 1987)"
     primer_bind     complement(3833..3849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3857..3873)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3881..3911)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3926..3947)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4235..4823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4997..5854)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5855..5959)
                     /label=AmpR promoter
ORIGIN
        1 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
       61 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
      121 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
      181 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      241 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      301 acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccttgtat ctctacacac
      361 aggctcaaat caataagaag aacggttcgt ctttttcgtt tatatcttgc atcgtcccaa
      421 agctattggc gggatattct gtttgcagtt ggctgacttg aagtaatctc tgcagatctt
      481 tcgacactga aatacgtcga gcctgctccg cttggaagcg gcgaggagcc tcgtcctgtc
      541 acaactacca acatggagta cgataagggc cagttccgcc agctcattaa gagccagttc
      601 atgggcgttg gcatgatggc cgtcatgcat ctgtacttca agtacaccaa ccctcttctg
      661 atccagtcga tcatcccgct gaagggcgct ttcgaatcga atctggttaa gatccacgtc
      721 ttcgggaagc cagcgactgg tgacctccag cgtcccttta aggctgccaa cagctttctc
      781 agccagggcc agcccaagac cgacaaggcc tccctccaga acgccgagaa gaactggagg
      841 ggtggtgtca aggaggagta agctccttat tgaagtcgga ggacggagcg gtgtcaagag
      901 gatattcttc gctctgtatt atagataaga tgatgaggaa ttggaggtag catagcttca
      961 tttggatttg ctttccaggc tgagactcta gcttggagca tagagggtcc ctttggcttt
     1021 caatattctc aagtatctcg agtttgaact tattcccgtg aaccttttat tcaccaatga
     1081 gcattggaat gaacatgaat ctgaggactg caatcgccat gaggttttcg aaatacatcc
     1141 ggatgtcgaa ggcttggggc acctgcgttg gttgaattta gaacgtggca ctattgatca
     1201 tccgatagct ctgcaaaggg cgttgcacaa tgcaagtcaa acgttgctag cagttccagg
     1261 tggaatgtta tgatgagcat tgtattaaat caggagatat agcatgatct ctagttagct
     1321 caccacaaaa gtcagacggc gtaaccaaaa gtcacacaac acaagctgta aggatttcgg
     1381 cacggctacg gaagacggag aagcccacct tcagtggact cgagtaccat ttaattctat
     1441 ttgtgtttga tcgagaccta atacagcccc tacaacgacc atcaaagtcg tatagctacc
     1501 agtgaggaag tggactcaaa tcgacttcag caacatctcc tggataaact ttaagcctaa
     1561 actatacaga ataagatggt ggagagctta taccgagctc ccaaatctgt ccagatcatg
     1621 gttgaccggt gcctggatct tcctatagaa tcatccttat tcgttgacct agctgattct
     1681 ggagtgaccc agagggtcat gacttgagcc taaaatccgc cgcctccacc atttgtagaa
     1741 aaatgtgacg aactcgtgag ctctgtacag tgaccggtga ctctttctgg catgcggaga
     1801 gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag acagctctgg
     1861 cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc cgccccgaag
     1921 tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg agaatatgga
     1981 gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt gtatagccgt
     2041 cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg atctggtaaa
     2101 agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc gcgtaagctc
     2161 cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc ctgctttgcc
     2221 cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac cgggaacaca
     2281 agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc tcagtccctg
     2341 gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca aggtcgttgc
     2401 gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct cttttctttt
     2461 ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt tatttcccct
     2521 aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc ttggaacctt
     2581 tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc ttgagcagac
     2641 atcaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     2701 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     2761 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     2821 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     2881 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     2941 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     3001 gtggccgagg agcaggactg attccggatc cacttaacgt tactgaaatc atcaaacagc
     3061 ttgacgaatc tggatataag atcgttggtg tcgatgtcag ctccggagtt gagacaaatg
     3121 gtgttcagga tctcgataag atacgttcat ttgtccaagc agcaaagagt gccttctagt
     3181 gatttaatag ctccatgtca acaagaataa aacgcgtttc gggtttacct cttccagata
     3241 cagctcatct gcaatgcatt aatgcattgg acctcgcaac cctagtacgc ccttcaggct
     3301 ccggcgaagc agaagaatag cttagcagag tctattttca ttttcgggag acgagatcaa
     3361 gcagatcaac ggtcgtcaag agacctacga gactgaggaa tccgctcttg gctccacgcg
     3421 actatatatt tgtctctaat tgtactttga catgctcctc ttctttactc tgatagcttg
     3481 actatgaaaa ttccgtcacc agcccctggg ttcgcaaaga taattgcact gtttcttcct
     3541 tgaactctca agcctacagg acacacattc atcgtaggta taaacctcga aaatcattcc
     3601 tactaagatg ggtatacaat agtaaccatg catggttgcc tagtgaatgc tccgtaacac
     3661 ccaatacgcc ggccgaaact tttttacaac tctcctatga gtcgtttacc cagaatgcac
     3721 aggtacactt gtttagaggt aatccttctt tctagaagtc ctcgtgtact gtgtaagcgc
     3781 ccactccaca tctccactcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
     3841 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3901 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     3961 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     4021 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     4681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5281 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     5341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5581 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6001 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
