LOCUS V020366 7377 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020366 VERSION V020366 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers repeat_region 1..141 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" enhancer 221..524 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 525..727 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 876..1254 /note="fusion between human cytomegalovirus intron A and human β-globin intron 2" /label="chimeric intron" CDS 1338..2045 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 2046..2102 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from porcine teschovirus-1 polyprotein" /transl_table=1 /translation="ATNFSLLKQAGDVEENPGP" /label="P2A" CDS 2109..4061 /label="Pias1(NM_019663)" /note="Pias1(NM_019663)" /gene="Pias1" CDS 4062..4085 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" polyA_signal 4124..4600 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" repeat_region 4640..4780 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" rep_origin 4855..5310 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5592..5696 /gene="bla" /label="AmpR promoter" CDS 5697..6557 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 6728..7316 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg 1381 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg 1441 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 1501 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca 1561 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg 1621 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg 1681 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc 1741 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga 1801 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg 1861 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc 1921 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca 1981 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt 2041 acaaggccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa aaccccggtc 2101 ctggatccat ggcggacagt gcggaactaa agcaaatggt tatgagcctt agagtttctg 2161 aactccaagt actgttgggc tacgctggga ggaacaagca cggacgcaaa cacgaacttc 2221 ttacaaaagc cctgcatttg ttaaaggctg gctgtagtcc tgctgtacaa atgaaaatta 2281 aagaactcta caggaggcgg ttccctcaga aaattatgac gcctgcggac ttgtctatcc 2341 ccaacgtaca ttcaagtcct atgcctccga ctctttctcc atccaccatt ccacagctca 2401 cttatgatgg ccaccctgca tcatccccac tactccctgt ttctcttctg ggacccaaac 2461 atgaactgga actcccacat ctcacgtcag cgctgcaccc agtccacccg gacataaagc 2521 tgcagaagct accattctat gacctgttgg atgaactgat caagcccacc agtctagctt 2581 cagacaacag ccagcgcttt cgggaaacct gttttgcatt tgccttgaca ccacaacagg 2641 tgcagcagat cagcagctcc atggatattt ctgggaccaa atgtgacttc acagtgcagg 2701 tccaattaag gttttgttta tcagaaacca gttgtccaca agaagatcac ttcccaccca 2761 acctttgtgt aaaagtgaat acaaaacctt gcagccttcc aggttacctt ccacctacta 2821 aaaacggtgt ggaaccaaag cgacctagcc gaccaattaa tatcacctca cttgtccgat 2881 tgtccacgac agtaccaaat accattgttg tttcttggac tgcagaaatt ggaagaacct 2941 attccatggc agtatatctt gtaaaacagt tgtcctcaac agttcttctt cagaggttac 3001 gagcaaaggg aataaggaat ccggatcatt ctagagcttt aattaaagag aagttaactg 3061 cagatccaga tagtgagata gctactacca gcctacgggt ttcgctgctg tgtccacttg 3121 ggaaaatgcg actgacaatc ccctgtcggg cacttacctg ctcccacctt cagtgttttg 3181 atgcaactct ttacattcaa atgaatgaga aaaaaccaac atgggtttgt cctgtctgtg 3241 ataagaaggc cccatatgaa caccttatta ttgacgggtt gtttatggaa attctaaagt 3301 actgcacaga ctgtgacgag atacagttta aggaggatgg ctcgtgggct ccaatgaggt 3361 caaagaagga ggttcaagaa gtcactgcct cctacaatgg agttgatggt tgcttgagct 3421 ccacattgga gcatcaggta gcgtcccaca accagtcctc aaataaaaac aagaaagtcg 3481 aggtcattga cctaaccatt gacagctcgt cagatgaaga ggaggaagaa ccccctgcca 3541 agaggacctg tccttccctg tctcctacgt caccactaag taataaaggc attttaagtc 3601 ttcctcatca agcctcgcct gtgtcccgca ccccaagcct tcctgctgta gatacaagct 3661 acatcaacac ctccctcatc caggactaca ggcacccctt ccacatgacg cctatgcctt 3721 atgacttaca aggattagat ttctttcctt tcttatcagg agacaatcag cattacaaca 3781 cctccctgct agccgcagct gcagcggcgg tctcagatga ccaggacctc ctgcactcct 3841 cccggttttt cccgtatacc tcctcgcaga tgtttctcga ccagctaagt gcaggaggga 3901 gcacatctct gccagccacc aacggaagca gtagcggcag caacagcagc cttgtgtctt 3961 ccaacagtct gagagagagc catggccatg gtgtggccag caggagcagc gcagacacag 4021 cgtccatctt tggcatcata ccagacatta tctcattgga cgattacaag gacgacgatg 4081 acaagtgaag cttgcctcga gcagcgctgc tcgagagatc tacgggtggc atccctgtga 4141 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt 4201 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg 4261 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg 4321 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc 4381 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg 4441 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc 4501 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct 4561 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac 4621 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc 4681 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 4741 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt 4801 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 4861 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 4921 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 4981 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 5041 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 5101 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 5161 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 5221 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 5281 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 5341 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 5401 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 5461 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 5521 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 5581 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 5641 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 5701 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 5761 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 5821 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 5881 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 5941 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 6001 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 6061 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 6121 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 6181 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 6241 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 6301 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 6361 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 6421 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 6481 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 6541 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 6601 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 6661 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 6721 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 6781 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 6841 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 6901 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6961 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 7021 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 7081 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 7141 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 7201 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 7261 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 7321 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt //