LOCUS V042626 5310 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V042626 VERSION V042626 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers repeat_region 1..141 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" enhancer 236..539 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 540..743 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 875..884 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 893..1336 /label="Rnase4(NM_021472)" /note="Rnase4(NM_021472)" /gene="Rnase4" CDS 1343..1414 /codon_start=1 /product="three tandem FLAG® epitope tags" /transl_table=1 /translation="DYKDDDDKDYKDDDDKDYKDDDDK" /label="3xFLAG" misc_feature 1437..2025 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2057..2533 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" repeat_region 2573..2713 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" rep_origin 2788..3243 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3525..3629 /gene="bla" /label="AmpR promoter" CDS 3630..4490 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 4661..5249 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagaacgcgt ggagctagtt 181 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta 241 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 301 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 361 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta 421 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 481 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg 541 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc 601 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact 661 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt 721 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc 781 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg taccggaatt 841 cggaactgga ggtggaggta gtggaatgga tcccgccacc atggcggccg ccatgatgga 901 tctacagagg actcagtcct tgcttctgct cttggtgctg accctgctgg ggttagggct 961 tgtacagccc tcctatggcc aggatcgaat gtaccaacgg ttccttcgac agcatgtgga 1021 ccctcaggtg acaggtggca atgacaacta ctgcaacgtg atgatgcaga gacggaagat 1081 gacttctgtc cagtgcaaac gcttcaacac cttcatccac gaagacatct ggaacattcg 1141 tggcatctgc agtaccacca atatcctgtg caagaacggc cagatgaact gtcacgaagg 1201 tgtagtgaag gtcacggact gcagagagac agggaactcc aaggccccca actgtagata 1261 cagggcaaga accagcacta ggcgagttgt cattgcctgt gagggtgacc cagaggtccc 1321 agtgcacttt gacagagcta gcgactacaa ggatgacgat gacaaggatt acaaagacga 1381 cgatgataag gactataagg atgatgacga caaaaagctt taaaccggtt atcgataatc 1441 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 1501 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 1561 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 1621 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 1681 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 1741 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 1801 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 1861 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 1921 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 1981 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc 2041 tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct 2101 ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt 2161 gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg 2221 gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc 2281 agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc 2341 tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt 2401 tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca 2461 ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc 2521 cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc 2581 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga 2641 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc 2701 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 2761 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg 2821 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 2881 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 2941 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 3001 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 3061 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 3121 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 3181 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 3241 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac 3301 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 3361 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 3421 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 3481 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 3541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 3601 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 3661 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 3721 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 3781 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 3841 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 3901 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 3961 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 4021 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 4081 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 4141 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 4201 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 4261 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 4321 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 4381 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 4441 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 4501 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 4561 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 4621 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 4681 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 4741 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 4801 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 4861 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 4921 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 4981 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 5041 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 5101 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 5161 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 5221 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 5281 tggccttttg ctggcctttt gctcacatgt //