LOCUS V032728 6344 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V032728 VERSION V032728 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers repeat_region 1..141 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" enhancer 156..535 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 536..739 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 770..1486 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1523..2443 /label="Nmnat2(NM_001048042)" /note="Nmnat2(NM_001048042)" /gene="Nmnat2" misc_feature 2471..3059 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3091..3567 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" repeat_region 3607..3747 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" rep_origin 3822..4277 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4559..4663 /gene="bla" /label="AmpR promoter" CDS 4664..5524 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 5695..6283 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtgacat tgattattga ctagttatta 181 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 481 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 541 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 601 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 661 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 721 ggtctatata agcagagctg gtaccggatc ctctagagtc gacgccacca tggtgagcaa 781 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 841 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 901 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 961 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 1021 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 1081 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 1141 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 1201 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 1261 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 1321 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 1381 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 1441 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggcg gtggaggcag 1501 tggtggaggc ggtagcgagc tcatgaccga gaccacaaag acccacgtta tcctgctggc 1561 ctgcggcagc ttcaatccca tcactaaagg gcacattcag atgttcgaaa gagccaggga 1621 ttatctgcac aagactggaa gatttattgt gattggtggt attgtctctc cagtccacga 1681 ctcctatgga aaacagggtc ttgtgtcaag tcggcaccgt ctcatcatgt gtcagctggc 1741 tgtccaaaat tctgactgga tcagggtgga cccatgggag tgctaccagg acacctggca 1801 gacaacctgc agtgtgttag agcaccatcg agacctcatg aagagggtga ccggctgcat 1861 cctctccaac gtcaacacgc cttccatgac acctgtgatt ggacagccac aacacgagaa 1921 cacccagccc atttaccaga acagcaatgt gcccaccaag cccactgcag ccaagatctt 1981 gggaaaggtg ggagagagcc tcggccggat ctgctgtgtc cgtccacccg tggagcgctt 2041 cacttttgta gatgagaacg ccaacctggg cacagtgatg cggtatgagg agatcgagct 2101 gcgcatcttg ctgctgtgcg gtagtgacct gctggagtcc ttctgcatcc caggactctg 2161 gaatgaggca gatatggaag tgattgttgg ggattttggg attgtggtgg tgccccggga 2221 tgcagcggac gcagaccgaa tcatgaatca ctcctcaata ctccgcaaat acaaaaacaa 2281 catcatggta gtgaaggatg acatcaacca tcccatgtct gtagtcagct ccaccaaaag 2341 caggctggcc ctgcagcatg gggatggcca cgttgtggat tacctgtccc agccggtcat 2401 cgactacatc ctcaagagtc agctgtacat caatgcctcg ggctaggaat tcgatatcaa 2461 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 2521 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 2581 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 2641 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 2701 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 2761 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 2821 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc 2881 ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 2941 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 3001 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca 3061 tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg 3121 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 3181 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 3241 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 3301 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 3361 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 3421 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 3481 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 3541 tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc 3601 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca 3661 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga 3721 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc 3781 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc 3841 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 3901 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 3961 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 4021 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt 4081 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 4141 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc 4201 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 4261 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 4321 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc 4381 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc 4441 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt 4501 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 4561 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 4621 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 4681 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 4741 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 4801 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 4861 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 4921 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 4981 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 5041 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 5101 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 5161 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 5221 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 5281 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 5341 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 5401 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 5461 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 5521 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 5581 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 5641 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 5701 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 5761 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 5821 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 5881 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 5941 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 6001 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 6061 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 6121 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 6181 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 6241 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 6301 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt //