LOCUS       V026663                 7527 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026663
VERSION     V026663
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     enhancer        156..535
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        536..739
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             758..2857
                     /label="Lhcgr(NM_013582)"
                     /note="Lhcgr(NM_013582)"
                     /gene="Lhcgr"
     CDS             2873..2926
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             2936..3631
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Zoanthus green fluorescent protein"
                     /transl_table=1
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP*"
                     /label="ZsGreen1"
     misc_feature    3654..4242
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4274..4750
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     repeat_region   4790..4930
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      5005..5460
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5742..5846
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5847..6707
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6878..7466
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgacat tgattattga ctagttatta
      181 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      481 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      541 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      601 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      661 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      721 ggtctatata agcagagctg gtaccggatc cgccaccatg gggcggcggg tcccggctct
      781 gagacagctg ctggtgctgg caatgctggt gctgaagcag tcacagctgc actctccaga
      841 gttgtcaggg tcgcgctgcc ctgagccctg cgactgcgcg ccggatggtg ccctgcgctg
      901 ccctggccct cgagctggcc tcgcccgact atctctcacc tatctccctg tcaaagtaat
      961 cccatcacaa gctttcaggg gacttaatga ggtcgtaaaa attgaaatct ctcagagtga
     1021 ttccctggaa aggatagaag ctaatgcctt tgacaacctc ctcaatctgt ctgaaatact
     1081 gatccagaac accaaaaacc tgctatacat tgaacccggt gcttttacaa acctccctcg
     1141 gttaaaatac ctgagcatct gtaacacagg catccggacc ctcccagatg tttcgaagat
     1201 ctcttcctct gaatttaatt tcattctgga aatctgtgat aacttataca taaccaccat
     1261 accagggaac gctttccaag ggatgaataa tgagtccatc acgctgaaac tgtatggaaa
     1321 tgggtttgaa gaagtacaaa gccatgcatt caatgggacg acgctaatct cgctggagtt
     1381 aaaagaaaac atctacctgg agaagatgca cagtggcacc ttccaggggg ccacggggcc
     1441 cagcatcctg gatgtctctt ccaccaaatt gcaggccctg ccgagccacg ggctggagtc
     1501 cattcagacg ctcatcgcca cgtcatccta ctcactgaaa actctgccct ccagagaaaa
     1561 attcaccagc ctactggttg ccacgctgac ctaccctagc cactgctgtg ctttcaggaa
     1621 tttgccgaag aaagaacaga atttttcatt ttccattttt gaaaactttt ccaaacaatg
     1681 tgaaagcaca gttagagaag cgaataacga gacgctttat tctgccatct ttgaggagaa
     1741 tgaactcagt ggctgggatt acgattatga cttctgttca cccaagacac tccaatgtac
     1801 tccagaacca gatgctttca atccctgtga agatattatg ggctatgcct tccttagggt
     1861 gttgatttgg ctaattaata tactagccat ctttggcaac ttgacagtcc tctttgttct
     1921 cctgaccagt cgttataaac tgacggtgcc ccgcttcctc atgtgtaatc tctcctttgc
     1981 agacttttgc atggggctct acctgctgct cattgcctca gtagactccc aaacaaaagg
     2041 ccagtactat aaccatgcca tagactggca gacagggagt ggctgcagtg cagctggctt
     2101 ctttactgtg ttcgccagtg aactttctgt ctataccctt acagtcatca ctctggaaag
     2161 gtggcacacc atcacctatg ctgttcagct ggaccaaaag ctgaggctga gacatgccat
     2221 cccaattatg ctcggaggat ggattttttc taccctgatg gccacattgc cccttgtggg
     2281 tgtcagcagt tacatgaaag tcagcatctg cctccccatg gatgtggaat ccactctgtc
     2341 acaagtctac atattatcca tcttgctcct caatgcagtg gcctttgtcg tcatctgtgc
     2401 ttgctacgtt aggatatact ttgcagttca aaatccagag ctgacggctc ctaacaagga
     2461 cacaaaaatt gctaagaaga tggccatcct catcttcaca gacttcacat gcatggcacc
     2521 catctcattc tttgccatct cagctgcctt caaagtaccc cttatcactg tcaccaactc
     2581 aaaagttctg ctggtccttt tttatcctgt caattcttgt gccaacccat ttctgtacgc
     2641 agtgttcacg aaggcatttc agagagattt ctttctcttg ctgagcagat ttggttgctg
     2701 taagcaccgg gctgaacttt acagaaggaa ggaattttct gcatgtacct tcaactccaa
     2761 aaacggcttt ccaagatcaa gtaagccttc ccaggctgcc ctgaagttat ccatagtgca
     2821 ctgtcaacaa cctacacctc caagagtgtt aattcagact ctagagtcga cagagggcag
     2881 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggcccttccg gaaccatggc
     2941 ccagtccaag cacggcctga ccaaggagat gaccatgaag taccgcatgg agggctgcgt
     3001 ggacggccac aagttcgtga tcaccggcga gggcatcggc taccccttca agggcaagca
     3061 ggccatcaac ctgtgcgtgg tggagggcgg ccccttgccc ttcgccgagg acatcttgtc
     3121 cgccgccttc atgtacggca accgcgtgtt caccgagtac ccccaggaca tcgtcgacta
     3181 cttcaagaac tcctgccccg ccggctacac ctgggaccgc tccttcctgt tcgaggacgg
     3241 cgccgtgtgc atctgcaacg ccgacatcac cgtgagcgtg gaggagaact gcatgtacca
     3301 cgagtccaag ttctacggcg tgaacttccc cgccgacggc cccgtgatga agaagatgac
     3361 cgacaactgg gagccctcct gcgagaagat catccccgtg cccaagcagg gcatcttgaa
     3421 gggcgacgtg agcatgtacc tgctgctgaa ggacggtggc cgcttgcgct gccagttcga
     3481 caccgtgtac aaggccaagt ccgtgccccg caagatgccc gactggcact tcatccagca
     3541 caagctgacc cgcgaggacc gcagcgacgc caagaaccag aagtggcacc tgaccgagca
     3601 cgccatcgcc tccggctccg ccttgccctg aattcgatat caagcttatc gataatcaac
     3661 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3721 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3781 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3841 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3901 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3961 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4021 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4081 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4141 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4201 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc
     4261 tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga
     4321 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc
     4381 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca
     4441 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt
     4501 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca
     4561 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt
     4621 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt
     4681 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt
     4741 ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta
     4801 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4861 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4921 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4981 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5041 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5101 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5161 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5221 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5281 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5341 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5401 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     5461 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5521 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5581 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5641 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5701 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5761 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5821 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5881 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5941 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6001 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6061 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6121 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6181 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6241 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6301 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6361 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6421 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6481 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6541 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6661 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6841 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6901 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6961 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7021 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7081 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7141 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7201 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7261 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7321 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7381 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7441 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7501 ccttttgctg gccttttgct cacatgt
//
