LOCUS       40924_1964        6370 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Integration vector mini-CTX-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
  TITLE     A site-specific gene integration system for Pseudomonas aeruginosa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-1999) Microbiology, Colorado State University, 
            Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 6370)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6370)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-APR-1999) Microbiology, Colorado State University, Fort Collins,
            CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        827..845
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     882..898
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(911..1624)
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1692..1708)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1734..1752)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(1926..1973)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     oriT            complement(2180..2288)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(3713..4301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5126..6313)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        complement(join(6361..6370,1..19))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 atcgatgata agctgtcaaa catgagaatt aattcatgtt ctttcctgcg ttatcccctg
       61 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      181 ctctccccgc gcgttggccg attcattaat gcagcgaatt agcttcaaaa gcgctctgaa
      241 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat cccctgattc
      301 cctttgtcaa cagcaatgga ttttcaatgg gtcaacatga aatccggctg ttgccctggg
      361 acggcaggtt tgacggcagg cttccaggct ctctcgcaca cgctgggaac actctgggaa
      421 caaacgcgag tgaaaatgga cagtagggcc caggaataat gcggcctcca gcgaggttgg
      481 ggcgtttcat atgagttcga atctcaccgc ctccgccata taacctgctg ttttcattga
      541 tgtttccatt cgacccactc tcaggagtga acgatttggg aacaaactgg gaacgaggcg
      601 cagaaaaaag gggccccgca gggccccttt ttcgttgctt caggaaagtt tcagcgcctg
      661 attcagcaga cctacgatgt cggggccgtc cttgctgaat tagctttatg cttgtaaacc
      721 gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca attaattagt
      781 aatataatct attaaaggtc attcaaaagg tcatccaccg gcgcgcaatt aaccctcact
      841 aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatcgt
      901 acgaatgcta tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact
      961 caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg
     1021 acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat
     1081 aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt
     1141 tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt
     1201 ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc
     1261 tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat
     1321 ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt
     1381 catgccgttt catatgatct gggtatcttg aaaagcattg aacaccataa gagaaagtag
     1441 tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa
     1501 gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa
     1561 catcaccatc taattcaaca agaattggga caactccagt gaaaagttct tctcctttac
     1621 tcatatgtat atctccttct taaatctaga ggatccccgg gtaccgagct cgaattcgat
     1681 atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt cgccctatag
     1741 tgagtcgtat tacgcgcgcc ggtggatgac cttttgaatg acctttaata gattatatta
     1801 ctaattaatt ggggacccta gaggtcccct tttttatttt aaaaattttt tcacaaaacg
     1861 gtttacaagc ataaagctaa ttccattgag taagttttta agcacatcag cttcaaaagc
     1921 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac ttcaagatcc
     1981 ccaattcgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2041 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2101 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2161 agaacatgct agagcttatc ggccagcctc gcagagcagg attcccgttg agcaccgcca
     2221 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
     2281 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
     2341 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga
     2401 agcagggtta tgcagcggaa agtatacctt aacctttttc gttgcttcag gaaagtttca
     2461 gcgcctgatt cagcagacct acgatgtcgg ggccgtcctt gctgatccac ttggcgtaat
     2521 gcttgaagat catcgccgtc gaggtgtgcc ccatctggtc cgcgatccat tcgggagtgg
     2581 cgatgccgct gctgagcatc tggctggcga aggtgtggcg gcactgattg ggaccacgcg
     2641 agcggacgcc cgcattcctg aggtggccat gccaccagcc gttgcgcagc gtatccgacg
     2701 tggagtacgc cgccccgcta gcactgttgt ggaacacgaa gcgtacccgt tgaggctttc
     2761 tggtccggtt gtcgcggtct atcacctcga tctgtacggg cggaaggcgt ctggttagct
     2821 tggcctgctg ctggagtgcg cgcagcgccg gggccagtag ctggaccttc ctcgtcgatc
     2881 ggcgagtctt ggtgaccttg tactggctgc gtacacgagc gcggcggaaa accacggtcc
     2941 ccgtgtccaa atcgacatct tcccaggcca gagctatggc ctcgctgacg cgggggccgc
     3001 tccagatcat gaactccgcc aggttcagct caccgatgcg cgcggtttca gtaccgagga
     3061 tgaggtcgat ttcctcacga gtgaatgggt ctgggtcatc cgcatccgga agagtgatca
     3121 cgatgccgtc tgttgggtcg tgcgcgctcc ggttccgagt tcgatacagc cggaagatct
     3181 gccgcaggtt gctgacgatc tctcgcaccg tcttgttgtg cagcttgggc atgagcgtgt
     3241 tctgcaccca gtcttgaata tccaggtggt cgatgctgtc ggcctggctg tcaccccagc
     3301 gaggtcggat gtgggtttcc acgcggctgg tgtagccgcg gaagccgctg gcggcaatct
     3361 ggttgcgttt gatgtcgagc cagaggtcga tgtaatggcc caaggtgttg cttttcaccc
     3421 gcggtgagtc cgggaagtgt cggctgtagc tgaagactcc ttgcttgatc tcgtagttga
     3481 tgatgccggc cagccgttcg gcattggcta tattctcggg cgttgcgtcc cctggtatgg
     3541 actccctaca cagctcgccc tggtagcgga agtagatcct gatccgtttt ccgcgtacct
     3601 cgactccgtc tgccatctgg ttcccctgat ccacaccaag cgttcaagct ggcatgtgag
     3661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4021 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4081 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4141 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4201 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4261 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4321 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4381 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4441 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4501 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4561 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4621 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4681 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4741 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4801 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4861 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4921 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4981 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtcaagg gttggtttgc
     5041 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
     5101 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
     5161 acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg
     5221 tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc
     5281 tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
     5341 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
     5401 ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg
     5461 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     5521 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     5581 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     5641 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     5701 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcggcgc tctcccttat
     5761 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
     5821 caaggaatgg tgcatgtaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     5881 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     5941 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     6001 cgatgcgtcc ggcgtagaga atccacagga cgggtgtggt cgccatgatc gcgtagtcga
     6061 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg
     6121 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
     6181 agtgactggc gatgctgtcg gaatggacaa tatcccgcaa gaggcccggc agtaccggca
     6241 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat
     6301 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc
     6361 attaaagcta
//
