LOCUS       62056_23485        5551 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1076..2608
                     /codon_start=1
                     /label=VSV-G
                     /note="vesicular stomatitis virus G glycoprotein"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    complement(2665..2786)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2967..3422
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3754..3858
                     /label=AmpR promoter
     CDS             3859..4716
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4890..5478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattctg acactatgaa
     1081 gtgccttttg tacttagcct ttttattcat tggggtgaat tgcaagttca ccatagtttt
     1141 tccacacaac caaaaaggaa actggaaaaa tgttccttct aattaccatt attgcccgtc
     1201 aagctcagat ttaaattggc ataatgactt aataggcaca gccttacaag tcaaaatgcc
     1261 caagagtcac aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac
     1321 tacttgtgat ttccgctggt atggaccgaa gtatataaca cattccatcc gatccttcac
     1381 tccatctgta gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa
     1441 tccaggcttc cctcctcaaa gttgtggata tgcaactgtg acggatgccg aagcagtgat
     1501 tgtccaggtg actcctcacc atgtgctggt tgatgaatac acaggagaat gggttgattc
     1561 acagttcatc aacggaaaat gcagcaatta catatgcccc actgtccata actctacaac
     1621 ctggcattct gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat
     1681 caccttcttc tcagaggacg gagagctatc atccctggga aaggagggca cagggttcag
     1741 aagtaactac tttgcttatg aaactggagg caaggcctgc aaaatgcaat actgcaagca
     1801 ttggggagtc agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc
     1861 tgcagccaga ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc
     1921 agtggatgta agtctaattc aggacgttga gaggatcttg gattattccc tctgccaaga
     1981 aacctggagc aaaatcagag cgggtcttcc aatctctcca gtggatctca gctatcttgc
     2041 tcctaaaaac ccaggaaccg gtcctgcttt caccataatc aatggtaccc taaaatactt
     2101 tgagaccaga tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat
     2161 gatcagtgga actaccacag aaagggaact gtgggatgac tgggcaccat atgaagacgt
     2221 ggaaattgga cccaatggag ttctgaggac cagttcagga tataagtttc ctttatacat
     2281 gattggacat ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgttcga
     2341 acatcctcac attcaagacg ctgcttcgca acttcctgat gatgagagtt tattttttgg
     2401 tgatactggg ctatccaaaa atccaatcga gcttgtagaa ggttggttca gtagttggaa
     2461 aagctctatt gcctcttttt tctttatcat agggttaatc attggactat tcttggttct
     2521 ccgagttggt atccatcttt gcattaaatt aaagcacacc aagaaaagac agatttatac
     2581 agacatagag atgaaccgac ttggaaagta actcaaatcc tgcactctag agtcgacccg
     2641 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     2701 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2761 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2821 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     2881 tcgataagga tccgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2941 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3001 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3061 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3121 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3181 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3241 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3301 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3361 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     3421 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     3481 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     3541 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     3601 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     3661 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3721 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3841 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3901 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3961 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4021 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4081 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4141 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4201 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4261 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4321 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4381 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4441 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4501 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4561 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4621 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4681 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4741 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4801 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5281 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5461 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5521 tggccttttg ctcacatggc tcgacagatc t
//
