LOCUS       V021291                 7662 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021291
VERSION     V021291
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     728..744
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     promoter        1442..1645
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             1734..2954
                     /label="TACR1(NM_001058)"
                     /note="TACR1(NM_001058)"
                     /gene="TACR1"
     promoter        2999..3210
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             3223..3491
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             3526..4125
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    4233..4354
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5641..5657)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5665..5681
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5689..5719)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5734..5755
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6043..6631)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6802..7662)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtattatg cccagtacat
     1381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1621 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     1681 tccacgctgt tttgacctcc atagaagatt ctagagctag cgaattcgcc accatggata
     1741 acgtcctccc ggtggactca gacctctccc caaacatctc cactaacacc tcggaaccca
     1801 atcagttcgt gcaaccagcc tggcaaattg tcctttgggc agctgcctac acggtcattg
     1861 tggtgacctc tgtggtgggc aacgtggtag tgatgtggat catcttagcc cacaaaagaa
     1921 tgaggacagt gacgaactat tttctggtga acctggcctt cgcggaggcc tccatggctg
     1981 cattcaatac agtggtgaac ttcacctatg ctgtccacaa cgaatggtac tacggcctgt
     2041 tctactgcaa gttccacaac ttctttccca tcgccgctgt cttcgccagt atctactcca
     2101 tgacggctgt ggcctttgat aggtacatgg ccatcataca tcccctccag ccccggctgt
     2161 cagccacagc caccaaagtg gtcatctgtg tcatctgggt cctggctctc ctgctggcct
     2221 tcccccaggg ctactactca accacagaga ccatgcccag cagagtcgtg tgcatgatcg
     2281 aatggccaga gcatccgaac aagatttatg agaaagtgta ccacatctgt gtgactgtgc
     2341 tgatctactt cctccccctg ctggtgattg gctatgcata caccgtagtg ggaatcacac
     2401 tatgggccag tgagatcccc ggggactcct ctgaccgcta ccacgagcaa gtctctgcca
     2461 agcgcaaggt ggtcaaaatg atgattgtcg tggtgtgcac cttcgccatc tgctggctgc
     2521 ccttccacat cttcttcctc ctgccctaca tcaacccaga tctctacctg aagaagttta
     2581 tccagcaggt ctacctggcc atcatgtggc tggccatgag ctccaccatg tacaacccca
     2641 tcatctactg ctgcctcaat gacaggttcc gtctgggctt caagcatgcc ttccggtgct
     2701 gccccttcat cagcgccggc gactatgagg ggctggaaat gaaatccacc cggtatctcc
     2761 agacccaggg cagtgtgtac aaagtcagcc gcctggagac caccatctcc acagtggtgg
     2821 gggcccacga ggaggagcca gaggacggcc ccaaggccac accctcgtcc ctggacctga
     2881 cctccaactg ctcttcacga agtgactcca agaccatgac agagagcttc agcttctcct
     2941 ccaatgtgct ctcctaggcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     3001 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     3061 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3121 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3181 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     3241 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     3301 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     3361 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     3421 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     3481 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgac cgagtacaag
     3541 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     3601 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     3661 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     3721 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     3781 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     3841 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     3901 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     3961 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     4021 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     4081 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     4141 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4201 ggatacgctg ctttaatgcc tttgtatcag ttaacttgtt tattgcagct tataatggtt
     4261 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4321 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggaattga ctcaaatgat
     4381 gtcaattagt ctatcagaag ctatctggtc tcccttccgg gggacaagac atccctgttt
     4441 aatatttaaa cagcagtgtt cccaaactgg gttcttatat cccttgctct ggtcaaccag
     4501 gttgcagggt ttcctgtcct cacaggaacg aagtccctaa agaaacagtg gcagccaggt
     4561 ttagccccgg aattgactgg attccttttt tagggcccat tggtatggct ttttccccgt
     4621 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
     4681 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
     4741 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
     4801 cgtaattaca tccctggggg ctttgggggg gggctgtccc tgatatctat aacaagaaaa
     4861 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     4921 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     4981 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     5041 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     5101 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctagc
     5161 tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct
     5221 atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga
     5281 aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca
     5341 tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca
     5401 cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc
     5461 gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta
     5521 atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc
     5581 cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg
     5641 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5701 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5761 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5821 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5881 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5941 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6001 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6061 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc at
//
