LOCUS       V026458                 8229 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026458
VERSION     V026458
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     728..744
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     promoter        1442..1645
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             1734..3518
                     /label="P2RX7(NM_002562)"
                     /note="P2RX7(NM_002562)"
                     /gene="P2RX7"
     promoter        3566..3777
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             3790..4058
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4093..4692
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    4800..4921
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6208..6224)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6232..6248
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6256..6286)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6301..6322
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6610..7198)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7369..8229)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtattatg cccagtacat
     1381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1621 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     1681 tccacgctgt tttgacctcc atagaagatt ctagagctag cgaattcgcc accatgccgg
     1741 cctgctgcag ctgcagtgat gttttccagt atgagacgaa caaagtcact cggatccaga
     1801 gcatgaatta tggcaccatt aagtggttct tccacgtgat catcttttcc tacgtttgct
     1861 ttgctctggt gagtgacaag ctgtaccagc ggaaagagcc tgtcatcagt tctgtgcaca
     1921 ccaaggtgaa ggggatagca gaggtgaaag aggagatcgt ggagaatgga gtgaagaagt
     1981 tggtgcacag tgtctttgac accgcagact acaccttccc tttgcagggg aactctttct
     2041 tcgtgatgac aaactttctc aaaacagaag gccaagagca gcggttgtgt cccgagtatc
     2101 ccacccgcag gacgctctgt tcctctgacc gaggttgtaa aaagggatgg atggacccgc
     2161 agagcaaagg aattcagacc ggaaggtgtg tagtgtatga agggaaccag aagacctgtg
     2221 aagtctctgc ctggtgcccc atcgaggcag tggaagaggc cccccggcct gctctcttga
     2281 acagtgccga aaacttcact gtgctcatca agaacaatat cgacttcccc ggccacaact
     2341 acaccacgag aaacatcctg ccaggtttaa acatcacttg taccttccac aagactcaga
     2401 atccacagtg tcccattttc cgactaggag acatcttccg agaaacaggc gataattttt
     2461 cagatgtggc aattcagggc ggaataatgg gcattgagat ctactgggac tgcaacctag
     2521 accgttggtt ccatcactgc cgtcccaaat acagtttccg tcgccttgac gacaagacca
     2581 ccaacgtgtc cttgtaccct ggctacaact tcagatacgc caagtactac aaggaaaaca
     2641 atgttgagaa acggactctg ataaaagtct tcgggatccg ttttgacatc ctggtttttg
     2701 gcaccggagg aaaatttgac attatccagc tggttgtgta catcggctca accctctcct
     2761 acttcggtct ggccgctgtg ttcatcgact tcctcatcga cacttactcc agtaactgct
     2821 gtcgctccca tatttatccc tggtgcaagt gctgtcagcc ctgtgtggtc aacgaatact
     2881 actacaggaa gaagtgcgag tccattgtgg agccaaagcc gacattaaag tatgtgtcct
     2941 ttgtggatga atcccacatt aggatggtga accagcagct actagggaga agtctgcaag
     3001 atgtcaaggg ccaagaagtc ccaagacctg cgatggactt cacagatttg tccaggctgc
     3061 ccctggccct ccatgacaca cccccgattc ctggacaacc agaggagata cagctgctta
     3121 gaaaggaggc gactcctaga tccagggata gccccgtctg gtgccagtgt ggaagctgcc
     3181 tcccatctca actccctgag agccacaggt gcctggagga gctgtgctgc cggaaaaagc
     3241 cgggggcctg catcaccacc tcagagctgt tcaggaagct ggtcctgtcc agacacgtcc
     3301 tgcagttcct cctgctctac caggagccct tgctggcgct ggatgtggat tccaccaaca
     3361 gccggctgcg gcactgtgcc tacaggtgct acgccacctg gcgcttcggc tcccaggaca
     3421 tggctgactt tgccatcctg cccagctgct gccgctggag gatccggaaa gagtttccga
     3481 agagtgaagg gcagtacagt ggcttcaaga gtccttactg ataggcggcc gcgaaggatc
     3541 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     3601 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     3661 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     3721 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     3781 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     3841 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     3901 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     3961 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     4021 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     4081 ctagacgcca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     4141 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     4201 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     4261 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     4321 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     4381 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     4441 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     4501 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     4561 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     4621 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     4681 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4741 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcagtta
     4801 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4861 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4921 atcatgtctg gaattgactc aaatgatgtc aattagtcta tcagaagcta tctggtctcc
     4981 cttccggggg acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt
     5041 cttatatccc ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag
     5101 tccctaaaga aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag
     5161 ggcccattgg tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     5221 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     5281 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     5341 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg
     5401 ctgtccctga tatctataac aagaaaatat atatataata agttatcacg taagtagaac
     5461 atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc
     5521 atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg
     5581 acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg
     5641 gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc
     5701 agactatctt tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc
     5761 ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct
     5821 tttcccgcga ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg
     5881 acgttgagcg aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct
     5941 ttaacggtga actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga
     6001 cacagttccg gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc
     6061 ggaactgccg tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca
     6121 gcgaggacgg gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac
     6181 ccggcgggcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7021 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     7081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8221 aatactcat
//
