LOCUS V041896 10176 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041896 VERSION V041896 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" promoter 2221..2432 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" intron 2433..3371 /note="intron upstream of the start codon of human EF-1α" /label="EF-1α intron A" regulatory 3387..3396 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 3396..3425 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 3426..4853 /label="Sec61a1(NM_016906)" /note="Sec61a1(NM_016906)" /gene="Sec61a1" misc_feature 4970..5519 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES" CDS 5522..6121 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6145..6733 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6940..7573 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7701..7717) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7725..7741 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7749..7779) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7794..7815 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8103..8691) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8862..9722) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9723..9827) /gene="bla" /label="AmpR promoter" polyA_signal 9875..10009 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt 2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc 2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt 2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg 2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 3361 ttccatttca ggtgtcgtga acgcgtgcca ccatggaaca aaaactcatc tcagaagagg 3421 atctgatggc gatcaaattt ctggaagtca tcaagccatt ctgtgtcatc ttgccggaaa 3481 ttcagaagcc agagagaaag attcagttta aggagaaagt tctgtggaca gccatcaccc 3541 tcttcatctt cttagtatgc tgtcagatcc ccctgtttgg catcatgtct tcagactcag 3601 ctgacccgtt ctactggatg cgagtgattc tggcctctaa cagaggtacc ttaatggagc 3661 tgggcatctc tcctattgtc acctccggtc ttatcatgca gctcttagct ggtgccaaga 3721 taattgaagt tggggacacc ccgaaagacc gagctctctt caatggagcc cagaagttgt 3781 ttggaatgat cattactatc ggtcagtcca ttgtgtatgt gatgacgggc atgtacgggg 3841 acccttcgga gatgggtgct ggaatctgcc tgctgatcac cattcagctc tttgttgctg 3901 gcttaattgt cctacttttg gatgaacttc tgcaaaaagg gtacggcttg ggctctggca 3961 tttctctctt cattgcgact aacatctgtg agaccattgt gtggaaggca ttcagcccca 4021 ccactgtcaa cactggccga gggatggaat tcgaaggcgc catcatagcg ctattccacc 4081 tgctggccac ccgcacagac aaggtccgag cccttcgaga ggcattctac cgccagaacc 4141 tccccaacct catgaacctg attgccacca tcttcgtctt tgccgttgtc atctatttcc 4201 agggcttccg agtggacctt ccaatcaagt cggcgcgtta ccgaggccag tacaacacct 4261 atcccatcaa gctcttctac acctccaaca tccccatcat cttgcagtct gctctggtgt 4321 ccaacctgta tgtcatctcc cagatgctgt cagcacgctt cagtggcaac ctgctggtta 4381 gcctgctggg cacctggtcg gatacatcat ctgggggccc agcccgagct taccccgttg 4441 gtggcctgtg ttactacctg tctcctccgg aatcgttcgg ctcagtgctg gaggaccctg 4501 tccatgcggt cgtgtacatc gtgttcatgc tgggctcctg tgcattcttc tccaaaacat 4561 ggatcgaagt ctcaggttct tctgccaaag atgttgcaaa gcagctgaag gagcagcaga 4621 tggtgatgag gggccacagg gagacctcca tggtgcatga gctcaaccgg tacatcccca 4681 cagccgcagc ctttggtggg ctgtgcattg gggccctctc tgtcctggct gacttcctgg 4741 gtgctatcgg gtctggaact gggatcctgc ttgcggtcac catcatctac cagtactttg 4801 agatcttcgt caaggaacag agcgaggtgg gcagcatggg agcccttctc ttctgagaat 4861 tcctcgaggg cggccgctct agagtcgacg ggccgcggta acaattgtta actaacttaa 4921 gctagcaacg gtttccctct agcgggatca attccgcccc ccccccctaa cgttactggc 4981 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 5041 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 5101 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 5161 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 5221 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 5281 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 5341 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 5401 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 5461 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac 5521 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 5581 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 5641 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 5701 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 5761 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 5821 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 5881 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 5941 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 6001 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 6061 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 6121 acccgggcgg ggcgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg 6181 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 6241 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 6301 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 6361 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 6421 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 6481 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 6541 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 6601 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 6661 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 6721 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca 6781 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 6841 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 6901 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc 6961 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 7021 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca 7081 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct 7141 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga 7201 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca 7261 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc 7321 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt 7381 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7441 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7501 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7561 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 7621 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc 7681 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 7741 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 7801 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 7861 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 7921 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 7981 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 8041 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 8101 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 8161 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 8221 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 8281 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 8341 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 8401 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 8461 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 8521 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 8581 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 8641 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 8701 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 8761 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 8821 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 8881 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 8941 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 9001 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 9061 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 9121 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 9181 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 9241 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 9301 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 9361 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 9421 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 9481 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 9541 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 9601 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 9661 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 9721 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 9781 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 9841 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg 9901 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 9961 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac 10021 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta 10081 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa 10141 ttgtatttgt taaatatgta ctacaaactt agtagt //