LOCUS       V040823                12210 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040823
VERSION     V040823
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2221..2432
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     intron          2433..3371
                     /note="intron upstream of the start codon of human EF-1α"
                     /label="EF-1α intron A"
     regulatory      3387..3396
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3396..3425
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3432..6887
                     /label="Adar(NM_019655)"
                     /note="Adar(NM_019655)"
                     /gene="Adar"
     misc_feature    7004..7553
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             7556..8155
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8179..8767
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8974..9607
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9735..9751)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9759..9775
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9783..9813)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9828..9849
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10137..10725)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10896..11756)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11757..11861)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11909..12043
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca ccatggaaca aaaactcatc tcagaagagg
     3421 atctgacgcg tatgtctcaa gggttcaggg gacccacagg ggtgttccct caccagacac
     3481 agtcgtactt ggaccctagt catgagcata gcaagtggag atacccgcag ccacaggggc
     3541 cggagtctta ccctaggagt ttccagcttc agcagataga gtttctcaaa gggcggctcc
     3601 cagaagcacc cttgattgga atacaaaccc agtcactgcc gccattcctc ccaggacact
     3661 ggccaagatt cccagggcca cctgcccaag acaggcaact ggaaatctgg gagttcccca
     3721 ggagtgtgac tctcagaaat caggggttcc acataggacc cccacttcct cccccacaca
     3781 gcaggggtac accatggaga ggtgctgacg ggctttgctc acacttccgg gagctgagca
     3841 tcagtcagag tccggagcag aaggttctaa accgcctgga agagcttggg gaggggaagg
     3901 ccaccactgc ccatgtgcta gccagagagc tcagaatccc caaaagggac atcaatcgta
     3961 ttttgtactc cctggaaaag aagggaaagc tgcacagagg aagggggaaa cctcctttgt
     4021 ggagccttgt gcccttgagt caggcttgga ctcagccccc tggagttgtg aatccagata
     4081 gttgtatcca ggaattccct agaggagagc ctggtttgga cagtgaggac ggagaccctg
     4141 cctctgactt agaaggacct tctgagcctc ttgacatggc tgaaatcaag gagaagatct
     4201 gtgactatct gttcaatgtg tcaaactcct ctgccctgaa cctggctaag aacattggcc
     4261 tcaccaaggc ccgagatgtg acctcagtgc tgattgactt ggaaaggcaa ggcgatgtct
     4321 acaggcaagg ggcaactcct cccatctggt acttgacgga caagaagcgt gagaggctgc
     4381 agatgaagag aagtacacac agtgctcctg cccctacccc gacagctgtc ccagaggcca
     4441 ctagaagccc ctcattccct gcctgccacc cgcccccagc aggtgcctca agcagtgtgg
     4501 cagcctccaa gagagtggag aatgggcagg agcctgcgat aaagcatgaa agtaggcatg
     4561 aggccagacc aggaccaatg agactgcggc ctcacgctta tcacaatggc ccctctagag
     4621 cagggtatgt ggcctctgaa aatggccagt gggccacaga tgacatccca gataacttga
     4681 atagtatcca cacagcacca ggtgagtttc gagccatcat ggagatgccc tccttctaca
     4741 gccctacctt gccacggtgt tcaccctaca agaagctaac tgagtgccag ctgaagaacc
     4801 ctgtcagcgg gttgttagag tatgctcagt tcactagtca gacctgtgat ttcaacctga
     4861 tagagcagag tggaccgtcc catgaacctc gatttaaatt ccaggttgtc atcaatgggc
     4921 gggaatttcc cccagctgag gctggcagca agaaagtagc caagcaggac gcagcagtga
     4981 aagccatggc gattctgctt cgggaagcca aagccaaaga cagtggtcaa ccagaagact
     5041 tgtcccactg tcccatggaa gaagactcgg agaaaccagc agaggctcag gcccccagct
     5101 cctcagcaac atccttgttc tctgggaaga gcccagttac tacactgctt gagtgcatgc
     5161 acaaactagg gaactcctgt gaattccgtc tcctgtccaa agaaggccct gctcatgacc
     5221 ccaagttcca gtactgtgta gcagtaggag cccagacctt cccccctgtg agcgccccca
     5281 gcaagaaggt agcaaagcag atggccgcag aggaagccat gaaggcgctg caggaggagg
     5341 cagccagttc agcggatgac cagtctggag gtgcgaacac agactcactt gatgaatcta
     5401 tggctcccaa caagatcagg aggattggtg agctcgtcag gtacctgaac accaaccccg
     5461 taggcggctt gttggagtac gcccgatctc atggctttgc tgctgagttc aagctcattg
     5521 accagtctgg acctcctcac gaacccaagt ttgtttacca agcaaaagtt gggggccgct
     5581 ggtttccagc cgtgtgtgca cacagcaaga aacagggcaa gcaggatgca gcggatgcag
     5641 ccctccgggt cttgatcggg gagagcgaga aggcagagca gttgggtttc gcagagcttc
     5701 ctctctctgg cagcaccttc cacgaccaga tagctatgct gagccacagg tgcttcaatg
     5761 ctctgaccaa cagtttccag ccctccctgc tcggccgcaa gatcctggct gccattatta
     5821 tgaaaagaga tccagaggac atgggtgttg tcgtgagttt ggggacaggc aatcgctgtg
     5881 tgaaagggga ctctctcagc ctgaagggag agacggtcaa tgactgccat gccgaaatca
     5941 tctcccggag gggcttcatc aggtttctct acagtgaact gatgaagtac aaccaccaca
     6001 ctgccaagaa cagcatattt gagcttgcca ggggaggaga gaagctgcag ataaagaaga
     6061 cggtttcttt tcatctctac atcagcacgg cgccatgtgg agatggggcc ctctttgaca
     6121 aatcctgcag tgaccgtgcc gtggaaagca cagagtcccg ccattaccct gtctttgaaa
     6181 atcccaagca aggcaagctt cgcaccaagg tggagaatgg ggaaggcaca attcctgtgg
     6241 agtccagtga tattgtaccc acgtgggatg gcatccgcct tggggaaaga ctccgtacca
     6301 tgtcctgtag tgacaaaatc ctacgctgga atgtgctggg cctgcaaggg gcgttgttga
     6361 cgcacttcct acagcctgtg tacctgaaat ctgtaacctt aggttacctt ttcagccaag
     6421 ggcatctgac ccgtgctatt tgctgccgcg tgaccagaga tgggaaagca tttgaggatg
     6481 gactacgcta tccctttatt gtcaaccacc ccaaggtcgg ccgagtcagt gtttatgatt
     6541 ccaaaagaca gtccggaaag accaaggaaa caagcgtcaa ctggtgcatg gctgatggct
     6601 atgacctaga gatcctggat ggcaccagag gcactgtgga tggaccaggg aaagagttgt
     6661 ctcgggtgtc caagaagaat attttccttc agtttaagaa gctctgctcc ttccgagccc
     6721 gcagagattt actgcagctc tcttatggtg aagccaagaa agctgcccgt gactatgact
     6781 tagccaagaa ctacttcaag aaaagcctgc gagacatggg ctatgggaat tggatcagca
     6841 aaccccagga ggaaaagaac ttttacctct gtccagtacc caatgactga gaattcctcg
     6901 agggcggccg ctctagagtc gacgggccgc ggtaacaatt gttaactaac ttaagctagc
     6961 aacggtttcc ctctagcggg atcaattccg cccccccccc ctaacgttac tggccgaagc
     7021 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     7081 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     7141 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     7201 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     7261 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     7321 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     7381 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     7441 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     7501 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatgac
     7561 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     7621 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     7681 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     7741 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     7801 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7861 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7921 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7981 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     8041 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     8101 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacccgg
     8161 gcggggcgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8221 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8281 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8341 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8401 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8461 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8521 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     8581 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8641 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8701 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8761 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     8821 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     8881 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     8941 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     9001 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     9061 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     9121 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     9181 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     9241 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     9301 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     9361 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     9421 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9481 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9541 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9601 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     9661 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
     9721 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9781 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9841 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9901 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9961 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10021 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10081 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10141 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10201 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10261 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10321 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10381 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10441 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10501 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10561 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10621 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10681 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10741 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10801 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10861 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10921 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10981 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11041 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11101 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11161 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11221 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11281 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11341 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11401 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11461 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11521 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11581 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11641 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11701 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11761 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11821 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11881 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
    11941 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    12001 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    12061 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    12121 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    12181 ttgttaaata tgtactacaa acttagtagt
//
