LOCUS       Exported                5463 bp DNA     circular SYN 08-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        688..1172
                     /label=Adenovirus TPL and MLP
                     /label=Adenovirus Tripartite Leader and Major Late
                     Promoter
                     /note="Regulatory DNA sequence from adenovirus containing
                     both the tripartite leader and major late promoter 
                     elements, functioning to initiate and enhance transcription
                     of late viral genes.; id: 3685243"
     sig_peptide     1236..1289
                     /product="human interleukin 10 signal peptide"
     CDS             1302..2312
                     /codon_start=1
                     /product="mouse IgG2b constant region"
                     /label=mouse IgG2b
                     /translation="AKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGS
                     LSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPSGPIS
                     TINPCPPCKECHKCPAPNLEGGPSVFIFPPNIKDVLMISLTPKVTCVVVDVSEDDPDVQ
                     ISWFVNNVEVHTAQTQTHREDYNSTIRVVSTLPIQHQDWMSGKEFKCKVNNKDLPSPIE
                     RTISKIKGLVRAPQVYILPPPAEQLSRKDVSLTCLVVGFNPGDISVEWTSNGHTEENYK
                     DTAPVLDSDGSYFIYSKLNMKTSKWEKTDSFSCNVRHEGLKNYYLKKTISRSPGK"
     polyA_site      2339..2394
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    2707..3434
                     /label=OriP
                     /note="Epstein-Barr virus (EBV) origin of replication"
     promoter        3563..3667
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3668..4528
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4699..5287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagagcg
     1201 gccgcaaact acaagacaga cttgcaaaag aaggcatgca cagctcagca ctgctctgtt
     1261 gcctggtcct cctgactggg gtgagggccg atatcggtac cgccaagaca acacctccta
     1321 gcgtgtaccc tctggctcct ggctgtggcg atacaacagg cagctctgtg acactgggct
     1381 gcctggtcaa gggctacttt cctgagagcg tgaccgtgac ctggaacagc ggaagcctgt
     1441 ctagcagcgt gcacacattc cctgctctgc tgcagtctgg cctgtacacc atgagcagca
     1501 gcgtgacagt gcccagcagc acatggccta gccagaccgt gacatgtagc gtggcccatc
     1561 ctgccagctc taccaccgtg gacaagaagc tggaacctag cggccccatc agcaccatca
     1621 atccctgtcc tccatgcaaa gaatgccaca agtgccccgc tcctaacctg gaaggtggcc
     1681 caagcgtgtt catcttccca cctaacatca aggacgtgct gatgatcagc ctgacaccta
     1741 aagtgacctg cgtggtggtg gacgtgtccg aggatgatcc cgatgtgcag atcagttggt
     1801 tcgtgaacaa cgtggaagtg cacacagccc agacacagac ccacagagag gactacaact
     1861 ccaccatcag agtggtgtcc acactgccta tccagcacca ggattggatg agcggcaaag
     1921 agttcaagtg caaagtgaac aacaaggacc tgccttctcc aatcgagcgg accatcagca
     1981 agatcaaggg actcgtcaga gcccctcagg tgtacatctt gcctccacca gccgagcagc
     2041 tgagcagaaa ggatgtgtcc ctgacctgtc tggtcgtggg cttcaatcct ggcgacatca
     2101 gcgtggaatg gaccagcaat ggccacaccg aggaaaacta caaggacaca gcccctgtgc
     2161 tggacagcga cggcagctac ttcatctaca gcaagctgaa catgaagacc agcaagtggg
     2221 agaaaaccga cagcttcagc tgcaacgtgc ggcacgaggg cctgaagaac tactacctga
     2281 agaaaaccat ctctcggagc cccggcaagt gaggatcccc cgacctcgac ctctggctaa
     2341 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     2401 aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct agctagagga
     2461 tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg
     2521 gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgaacc ctaaacgggt
     2581 agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata
     2641 tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac
     2701 agcgatgtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     2761 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     2821 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     2881 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     2941 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     3001 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     3061 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     3121 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     3181 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     3241 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     3301 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     3361 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     3421 atgctatcct catgataagc tgtcaaacat gagaattaat tcttgaagac gaaagggcct
     3481 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     3541 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3601 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3661 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3721 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3781 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     3841 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     3901 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3961 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4021 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4081 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4141 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4201 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4261 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4321 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4381 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4441 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4501 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4561 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4621 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4801 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5341 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     5401 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     5461 agc
//
