LOCUS       Exported                5427 bp DNA     circular SYN 08-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        688..1172
                     /label=Adenovirus TPL and MLP
                     /label=Adenovirus Tripartite Leader and Major Late
                     Promoter
                     /note="Regulatory DNA sequence from adenovirus containing
                     both the tripartite leader and major late promoter 
                     elements, functioning to initiate and enhance transcription
                     of late viral genes.; id: 3685243"
     sig_peptide     1236..1289
                     /product="human interleukin 10 signal peptide"
     CDS             1302..2276
                     /codon_start=1
                     /product="mouse IgG1 constant region"
                     /label=mouse IgG1
                     /translation="AKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGS
                     LSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPRDCGC
                     KPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHT
                     AQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKA
                     PQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMNTNGSY
                     FVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK"
     polyA_site      2303..2358
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    2671..3398
                     /label=OriP
                     /note="Epstein-Barr virus (EBV) origin of replication"
     promoter        3527..3631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3632..4492
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4663..5251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagagcg
     1201 gccgcaaact acaagacaga cttgcaaaag aaggcatgca cagctcagca ctgctctgtt
     1261 gcctggtcct cctgactggg gtgagggccg atatcggtac cgccaagaca acacctccta
     1321 gcgtgtaccc tctggctcct ggatctgccg ctcagaccaa tagcatggtc accctgggct
     1381 gtctggtcaa gggctacttt cctgagcctg tgaccgtgac ctggaacagc ggatctctgt
     1441 ctagcggcgt gcacaccttt ccagccgtgc tgcagagcga tctgtacacc ctgagcagca
     1501 gcgtgaccgt gcctagctct cctagaccta gcgagacagt gacctgcaac gtggcccatc
     1561 ctgccagcag caccaaggtg gacaagaaaa tcgtgcccag agactgcggc tgcaagccct
     1621 gcatctgtac cgtgcctgaa gtgtccagcg tgttcatctt cccacctaag cctaaggacg
     1681 tgctgaccat cacactgacc cctaaagtga cctgtgtggt ggtggacatc agcaaggacg
     1741 accccgaggt gcagttcagt tggttcgtgg acgacgtgga agtgcacaca gcccagacac
     1801 agcctagaga ggaacagttc aacagcacct tcagaagcgt gtccgagctg cccatcatgc
     1861 accaggattg gctgaacggc aaagagttca agtgcagagt gaacagcgcc gcctttcctg
     1921 ctcctatcga gaaaaccatc tccaagacca agggcagacc caaggctccc caggtgtaca
     1981 caatccctcc acctaaagaa cagatggcca aggacaaggt gtccctgacc tgcatgatca
     2041 ccgatttctt cccagaggac atcaccgtgg aatggcagtg gaatggacag cccgccgaga
     2101 actacaagaa cacccagcct atcatgaaca ccaacggcag ctacttcgtg tacagcaagc
     2161 tgaacgtgca gaagtccaac tgggaggccg gcaacacctt cacctgttct gtgctgcacg
     2221 agggcctgca caatcaccac accgagaagt ccctgagcca ctctcctggc aagtgaggat
     2281 cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     2341 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt ggtcgagatc
     2401 cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa cctgactacg
     2461 acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     2521 ttgccaactg aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
     2581 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
     2641 gttagtaaaa gggtcctaag gaacagcgat gtaggtgggc gggccaagat aggggcgcga
     2701 ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg
     2761 ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc
     2821 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag
     2881 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     2941 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     3001 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     3061 gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat
     3121 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta
     3181 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     3241 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     3301 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     3361 tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa acatgagaat
     3421 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3481 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3541 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3601 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3661 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3721 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3781 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3841 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     3901 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3961 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4021 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4081 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4141 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     4201 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4261 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4321 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4381 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4441 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4501 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4561 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4621 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4681 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4741 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4801 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4861 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4921 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4981 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5041 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5101 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5161 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5221 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5281 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5341 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5401 gcgcagcgag tcagtgagcg aggaagc
//
