LOCUS       Exported                5814 bp DNA     circular SYN 08-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        688..1172
                     /label=Adenovirus TPL and MLP
                     /label=Adenovirus Tripartite Leader and Major Late
                     Promoter
                     /note="Regulatory DNA sequence from adenovirus containing
                     both the tripartite leader and major late promoter 
                     elements, functioning to initiate and enhance transcription
                     of late viral genes.; id: 3685243"
     sig_peptide     1236..1289
                     /product="human interleukin 10 signal peptide"
     CDS             1302..2663
                     /codon_start=1
                     /product="immunoglobulin mu heavy chain"
                     /label=huIgM
                     /translation="GSASAPTLFPLVSCENSPSDTSSVAVGCLAQDFLPDSITFSWKYK
                     NNSDISSTRGFPSVLRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGNKEKNVPLP
                     VIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQ
                     VQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAI
                     RVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNAT
                     FSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQ
                     LNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILT
                     VSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY"
     polyA_site      2690..2745
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    3058..3785
                     /label=OriP
                     /note="Epstein-Barr virus (EBV) origin of replication"
     promoter        3914..4018
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4019..4879
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5050..5638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagagcg
     1201 gccgcaaact acaagacaga cttgcaaaag aaggcatgca cagctcagca ctgctctgtt
     1261 gcctggtcct cctgactggg gtgagggccg atatcggtac cggatctgcc tctgctccca
     1321 cactgttccc tctggtgtcc tgcgagaaca gccctagcga tacaagctct gtggccgtgg
     1381 gatgtctggc ccaggatttc ctgcctgaca gcatcacctt cagctggaag tacaagaaca
     1441 acagcgacat cagcagcacc cggggctttc catctgttct gagaggcggc aaatacgccg
     1501 ccacaagcca agtgctgctg cccagcaaag atgtgatgca gggcaccgat gagcacgtcg
     1561 tgtgcaaagt gcagcacccc aacggcaaca aagaaaagaa cgtgcccctg cctgtgatcg
     1621 ccgagctgcc tcctaaagtg tccgtgttcg tgcctcctcg cgacggcttc ttcggcaacc
     1681 ctagaaagag caagctgatc tgccaggcca ccggcttcag ccccagacag attcaagtgt
     1741 cctggctgcg cgagggcaag caagttggat ctggcgtgac caccgatcag gtgcaggccg
     1801 aggctaaaga gagcggccct accacctaca aagtgaccag cacactgacc atcaaagagt
     1861 ccgactggct gggccagtcc atgttcacct gtagagtgga ccaccggggc ctgacctttc
     1921 agcagaatgc cagctctatg tgcgtgcccg atcaggacac agccatccgg gtgttcgcca
     1981 ttcctcctag cttcgccagc atcttcctga ccaagagcac caagctgacc tgtctggtca
     2041 ccgacctgac cacctacgac agcgtgacaa tcagctggac caggcagaat ggcgaggccg
     2101 tgaaaaccca caccaacatc agcgagtctc accccaatgc cacctttagc gccgtgggcg
     2161 aagcctccat ttgcgaggac gattggaaca gcggcgagcg gtttacctgc accgtgacac
     2221 acaccgatct gccctctcca ctgaagcaga ccatcagcag acctaaaggc gtggccctgc
     2281 acagacccga tgtgtatttg ctgccaccag ccagagagca gctgaacctg agagagagcg
     2341 ccaccatcac ctgtctcgtg accggatttt cccctgccga tgtgtttgtg cagtggatgc
     2401 agagaggcca gcctctgagc cccgagaaat acgtgacaag cgcccctatg cctgagccac
     2461 aggcacccgg aagatacttt gcccacagca tcctgaccgt gtccgaggaa gaatggaata
     2521 ccggcgagac atacacctgt gtggtggccc atgaggccct gcctaataga gtgaccgagc
     2581 ggaccgtgga taagagcacc ggaaagccca ctctgtacaa cgtgtccctg gtcatgagcg
     2641 acaccgccgg cacatgttac tgaggatccc ccgacctcga cctctggcta ataaaggaaa
     2701 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     2761 gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg atcgatcccc
     2821 gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat
     2881 gtaatccctt cagttggttg gtacaacttg ccaactgaac cctaaacggg tagcatatgc
     2941 ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca
     3001 acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatgta
     3061 ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact
     3121 tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca
     3181 cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat agcatatgct
     3241 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     3301 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     3361 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     3421 atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct
     3481 atcctaattt atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc
     3541 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc
     3601 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     3661 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     3721 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     3781 tcatgataag ctgtcaaaca tgagaattaa ttcttgaaga cgaaagggcc tcgtgatacg
     3841 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     3901 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3961 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4021 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4081 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4141 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4201 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4261 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4321 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4381 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4441 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4501 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4561 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4621 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4681 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4741 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4801 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4861 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4921 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4981 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5161 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5461 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5641 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5701 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5761 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagc
//
