LOCUS       Exported                5436 bp DNA     circular SYN 08-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        688..1172
                     /label=Adenovirus TPL and MLP
                     /label=Adenovirus Tripartite Leader and Major Late
                     Promoter
                     /note="Regulatory DNA sequence from adenovirus containing
                     both the tripartite leader and major late promoter 
                     elements, functioning to initiate and enhance transcription
                     of late viral genes.; id: 3685243"
     sig_peptide     1236..1289
                     /product="human interleukin 10 signal peptide"
     CDS             1302..2285
                     /codon_start=1
                     /product="human IgG4 constant region"
                     /label=human IgG4 constant region
                     /translation="ASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGA
                     LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGP
                     PCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVE
                     VHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQ
                     PREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSD
                     GSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGK"
     polyA_site      2312..2367
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    2680..3407
                     /label=OriP
                     /note="Epstein-Barr virus (EBV) origin of replication"
     promoter        3536..3640
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3641..4501
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4672..5260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagagcg
     1201 gccgcaaact acaagacaga cttgcaaaag aaggcatgca cagctcagca ctgctctgtt
     1261 gcctggtcct cctgactggg gtgagggccg atatcggtac cgcctctaca aagggcccta
     1321 gtgtgttccc tctggctccc tgtagcagaa gcaccagcga atctacagcc gctctgggct
     1381 gcctggtcaa ggactacttt cctgagcctg tgaccgtgtc ctggaactct ggcgctctga
     1441 catctggcgt gcacaccttt ccagccgtgc tgcaaagcag cggcctgtac tctctgagca
     1501 gcgtggtcac agtgcctagc tctagcctgg gcaccaagac ctacacctgt aatgtggacc
     1561 acaagcctag caacaccaag gtggacaagc gcgtggaatc taagtacggc cctccttgtc
     1621 ctagctgccc cgctcctgaa tttctcggcg gaccttccgt gttcctgttt cctccaaagc
     1681 ctaaggacac cctgatgatc agcagaaccc ctgaagtgac ctgcgtggtg gtggacgttt
     1741 cccaagagga ccctgaggtg cagttcaatt ggtacgtgga cggcgtggaa gtgcacaacg
     1801 ccaagaccaa gcctagagag gaacagttca acagcaccta cagagtggtg tccgtgctga
     1861 cagtgctgca ccaggattgg ctgaacggca aagagtacaa gtgcaaggtg tccaacaagg
     1921 gcctgcctag cagcatcgag aaaaccatca gcaaggccaa gggccagcca agagaacccc
     1981 aggtgtacac actgcctcca agccaagagg aaatgaccaa gaaccaggtg tccctgacct
     2041 gcctcgtgaa gggcttttac cccagcgaca ttgccgtgga atgggagagc aatggccagc
     2101 ctgagaacaa ctacaagaca acccctcctg tgctggacag cgacggctca ttcttcctgt
     2161 acagcagact gaccgtggac aagagcagat ggcaagaggg caacgtgttc agctgcagcg
     2221 tgatgcacga ggccctgcac aaccactaca cccagaagtc tctgtccctg tctctgggca
     2281 agtgaggatc ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata
     2341 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg
     2401 gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga cgaactaaac
     2461 ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt
     2521 tggtacaact tgccaactga accctaaacg ggtagcatat gcttcccggg tagtagtata
     2581 tactatccag actaacccta attcaatagc atatgttacc caacgggaag catatgctat
     2641 cgaattaggg ttagtaaaag ggtcctaagg aacagcgatg taggtgggcg ggccaagata
     2701 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
     2761 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
     2821 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     2881 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     2941 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     3001 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     3061 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg
     3121 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     3181 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     3241 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     3301 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     3361 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata agctgtcaaa
     3421 catgagaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3481 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3601 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3661 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3721 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3781 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3841 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     3901 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3961 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4021 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4081 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4141 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     4201 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4261 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4321 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4381 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4441 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4501 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4561 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4621 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4681 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4741 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4801 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4861 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4921 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4981 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5041 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5101 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5161 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5221 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5281 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5341 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5401 aacgaccgag cgcagcgagt cagtgagcga ggaagc
//
