LOCUS       Exported                5445 bp DNA     circular SYN 08-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        688..1172
                     /label=Adenovirus TPL and MLP
                     /label=Adenovirus Tripartite Leader and Major Late
                     Promoter
                     /note="Regulatory DNA sequence from adenovirus containing
                     both the tripartite leader and major late promoter 
                     elements, functioning to initiate and enhance transcription
                     of late viral genes.; id: 3685243"
     sig_peptide     1236..1289
                     /product="human interleukin 10 signal peptide"
     CDS             1302..2294
                     /codon_start=1
                     /label=Human IgG1
                     /note="human IgG1 constant region"
                     /translation="ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA
                     LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD
                     KTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVD
                     GVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKA
                     KGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL
                     DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK"
     polyA_site      2321..2376
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    2689..3416
                     /label=OriP
                     /note="Epstein-Barr virus (EBV) origin of replication"
     promoter        3545..3649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3650..4510
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4681..5269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagagcg
     1201 gccgcaaact acaagacaga cttgcaaaag aaggcatgca cagctcagca ctgctctgtt
     1261 gcctggtcct cctgactggg gtgagggccg atatcggtac cgcctctaca aagggcccta
     1321 gtgtgttccc tctggctccc agcagcaagt ctacatctgg cggaacagcc gctctgggct
     1381 gcctggtcaa ggattacttt cccgagcctg tgaccgtgtc ctggaatagc ggagcactga
     1441 caagcggcgt gcacaccttt ccagctgtgc tgcaaagcag cggcctgtac tctctgagca
     1501 gcgtggtcac agtgcctagc tctagcctgg gcacccagac ctacatctgc aatgtgaacc
     1561 acaagcctag caacaccaag gtggacaaga aggtggaacc caagagctgc gacaagaccc
     1621 acacctgtcc tccatgtcct gctccagaac tgctcggcgg accttccgtg ttcctgtttc
     1681 ctccaaagcc taaggacacc ctgatgatca gcagaacccc tgaagtgacc tgcgtggtgg
     1741 tggatgtgtc tcacgaggac cccgaagtga agttcaattg gtacgtggac ggcgtggaag
     1801 tgcacaacgc caagaccaag cctagagagg aacagtacaa cagcacctac agagtggtgt
     1861 ccgtgctgac cgtgctgcac caggattggc tgaacggcaa agagtacaag tgcaaggtgt
     1921 ccaacaaggc cctgcctgct cctatcgaga aaaccatcag caaggccaag ggccagccta
     1981 gggaacccca ggtttacaca ctgcctccaa gcagggacga gctgaccaag aatcaggtgt
     2041 ccctgacctg cctcgtgaag ggcttttacc ccagcgacat tgccgtggaa tgggagagca
     2101 atggccagcc tgagaacaac tacaagacaa cccctcctgt gctggacagc gacggctcat
     2161 tcttcctgta cagcaagctg acagtggaca agtccagatg gcagcagggc aacgtgttca
     2221 gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagtcc ctgagcctgt
     2281 ctcctggcaa gtgaggatcc cccgacctcg acctctggct aataaaggaa atttattttc
     2341 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     2401 aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc cgccccggac
     2461 gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct
     2521 tcagttggtt ggtacaactt gccaactgaa ccctaaacgg gtagcatatg cttcccgggt
     2581 agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc
     2641 atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatgt aggtgggcgg
     2701 gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga
     2761 gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct
     2821 aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     2881 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     2941 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     3001 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     3061 tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt
     3121 tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     3181 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     3241 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     3301 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3361 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgataa
     3421 gctgtcaaac atgagaatta attcttgaag acgaaagggc ctcgtgatac gcctattttt
     3481 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3661 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3721 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3781 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3841 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     3901 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3961 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4021 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4081 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4141 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     4201 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4261 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4321 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4381 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4441 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4501 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4561 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5341 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5401 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagc
//
