LOCUS       Exported                5475 bp DNA     circular SYN 08-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        688..1172
                     /label=Adenovirus TPL and MLP
                     /label=Adenovirus Tripartite Leader and Major Late
                     Promoter
                     /note="Regulatory DNA sequence from adenovirus containing
                     both the tripartite leader and major late promoter 
                     elements, functioning to initiate and enhance transcription
                     of late viral genes.; id: 3685243"
     sig_peptide     1236..1289
                     /product="human interleukin 10 signal peptide"
     CDS             1302..2324
                     /codon_start=1
                     /product="Human Immunoglobulin heavy constant alpha 2"
                     /label=huIgA2
                     /translation="ASPTSPKVFPLSLDSTPQDGNVVVACLVQGFFPQEPLSVTWSESG
                     QNVTARNFPPSQDASGDLYTTSSQLTLPATQCPDGKSVTCHVKHYTNSSQDVTVPCRVP
                     PPPPCCHPRLSLHRPALEDLLLGSEANLTCTLTGLRDASGATFTWTPSSGKSAVQGPPE
                     RDLCGCYSVSSVLPGCAQPWNHGETFTCTAAHPELKTPLTANITKSGNTFRPEVHLLPP
                     PSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTYAV
                     TSILRVAAEDWKKGETFSCMVGHEALPLAFTQKTIDRMAGKPTHINVSVVMAEADGTCY
                     "
     polyA_site      2351..2406
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    2719..3446
                     /label=OriP
                     /note="Epstein-Barr virus (EBV) origin of replication"
     promoter        3575..3679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3680..4540
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4711..5299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagagcg
     1201 gccgcaaact acaagacaga cttgcaaaag aaggcatgca cagctcagca ctgctctgtt
     1261 gcctggtcct cctgactggg gtgagggccg atatcggtac cgcctctcct acaagcccca
     1321 aagtgttccc tctgagcctg gacagcacac cccaggatgg aaatgtggtg gtggcctgtc
     1381 tggtgcaggg attcttccca caagagcccc tgtccgtgac ttggagcgag agcggacaga
     1441 atgtgaccgc cagaaacttc ccacctagcc aggatgccag cggcgatctg tacacaacaa
     1501 gcagccagct gaccctgcct gccacacagt gtcctgatgg caagagcgtg acctgccacg
     1561 tgaagcacta caccaacagc tcccaggacg tgaccgtgcc ttgtagagtt cctcctcctc
     1621 caccttgctg tcaccccaga ctgtctctgc acagacccgc tctggaagat ctgctgctgg
     1681 gctctgaggc caacctgacc tgtacactga ccggcctgag agatgcctct ggcgccacct
     1741 ttacatggac acctagcagc ggaaagtccg ccgttcaggg acctcctgag agggatctgt
     1801 gtggctgtta cagcgtgtcc tctgtgctgc ctggatgtgc ccagccttgg aatcacggcg
     1861 agacattcac ctgtaccgcc gctcatcccg agctgaaaac acccctgacc gccaacatca
     1921 ccaagagcgg caacaccttc agacccgaag tgcatctgct gcctccacca tctgaagaac
     1981 tggccctgaa cgagctggtc accctgacat gtctggccag gggcttcagc cctaaggatg
     2041 tgctcgttag atggctgcag ggcagccaag agctgcccag agagaagtat ctgacctggg
     2101 ccagcagaca agagccaagc cagggcacaa caacctacgc cgtgaccagc attctgagag
     2161 tggccgccga ggattggaag aagggcgaaa ccttcagctg catggtcgga cacgaagccc
     2221 tgccactggc cttcacacag aaaaccatcg accggatggc cggcaagccc acacacatca
     2281 atgtgtccgt ggttatggcc gaggccgacg gcacatgtta ctgaggatcc cccgacctcg
     2341 acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     2401 ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc tcggagatct
     2461 ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc
     2521 cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactgaa
     2581 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     2641 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     2701 gtcctaagga acagcgatgt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct
     2761 ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata
     2821 ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca
     2881 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     2941 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     3001 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     3061 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata
     3121 gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata
     3181 tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3241 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3301 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     3361 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     3421 tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaatta attcttgaag
     3481 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     3541 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3601 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3661 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3721 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3781 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3841 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3901 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     3961 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4021 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4081 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4141 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4201 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4261 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4321 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4381 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4441 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4501 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4561 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4621 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4681 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4741 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4801 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4861 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4921 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4981 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5041 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5101 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5161 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5221 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5281 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5341 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5401 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5461 agtgagcgag gaagc
//
