LOCUS 40924_390 4138 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector CaMV35S-pFLEV, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4138) AUTHORS Zhang N, McHale LK, Finer JJ. TITLE Isolation and characterization of 'GmScream' promoters that regulate highly expressing soybean (Glycine max Merr.) genes JOURNAL Plant Sci. 241, 189-198 (2015) PUBMED 26706070 REFERENCE 2 (bases 1 to 4138) AUTHORS Zhang N. TITLE Direct Submission JOURNAL Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA REFERENCE 3 (bases 1 to 4138) TITLE Direct Submission REFERENCE 4 (bases 1 to 4138) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 241, 189-198 (2015)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4138 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 492..836 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 858..1574 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" terminator 1610..1862 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(1917..1933) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1941..1957) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1965..1995) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2010..2031) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2319..2907) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3081..3938) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3939..4043) /label=AmpR promoter ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctagag atccgtcaac 421 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 481 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 541 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 601 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 661 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 721 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 781 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacgacc 841 tgcaggtcga cggatccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 901 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 961 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 1021 ccgtgccctg gcccaccctc gtgaccacct tcacctacgg cgtgcagtgc ttcagccgct 1081 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 1141 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 1201 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 1261 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 1321 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 1381 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 1441 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 1501 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact cacggcatgg 1561 acgagctgta caagtaaagc ggccgcccgg ctgcactgag gcctcagcag atcgttcaaa 1621 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat 1681 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt 1741 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa 1801 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga 1861 tccgatgata agctgtcaaa catgagaatt agatctcaat tgaattcgta atcatggtca 1921 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 1981 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 2041 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 2101 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 2161 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 2221 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 2281 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 2341 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 2401 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 2461 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 2521 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 2581 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 2641 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 2701 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 2761 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 2821 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 2881 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 2941 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 3001 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 3061 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 3121 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 3181 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 3241 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 3301 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 3361 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 3421 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 3481 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 3541 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 3601 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 3661 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 3721 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 3781 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 3841 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 3901 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 3961 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 4021 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 4081 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc //