载体基本信息 | |||
载体名称 | pJH373 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5792 bp | 载体类型 | Mammalian Expression |
拷贝数 | High Copy | 启动子 | CMV |
克隆方法 | Gibson Cloning | 5'引物 | CMV-F |
3'引物 | BGH-Rev |
pJH373 载体载体图谱
pJH373 载体载体序列
LOCUS Exported 5792 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION mamalian expression of AcrIIA2. ACCESSION . VERSION . KEYWORDS pJH373 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5792) AUTHORS Rauch BJ, Silvis MR, Hultquist JF, Waters CS, McGregor MJ, Krogan NJ, Bondy-Denomy J TITLE Inhibition of CRISPR-Cas9 with Bacteriophage Proteins. JOURNAL Cell. 2016 Dec 22. pii: S0092-8674(16)31683-X. doi: 10.1016/j.cell.2016.12.009. PUBMED 28041849 REFERENCE 2 (bases 1 to 5792) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 Dec 22. pii: S0092-8674(16)31683-X. doi: 10.1016/j.cell.2016.12.009." FEATURES Location/Qualifiers source 1..5792 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 42..421 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 422..625 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 576..596 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 670..689 /label=T7 /note="T7 promoter, forward primer" promoter 670..688 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1190..1207) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1196..1420 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1466..1894 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1553..1572) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1763..1784 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(1903..1923) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1908..2237 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2088..2223 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2150..2169 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2304..3098 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2358..2377) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 2968..2987 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3272..3393 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3309..3328) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3363..3382 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3442..3458) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3442..3458) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3455..3477) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3466..3482 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3490..3520) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3535..3556 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3673..3690) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3844..4432) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3924..3943) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4603..5463) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5226..5245 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5464..5568) /gene="bla" /label=AmpR promoter primer_bind complement(5643..5662) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker"
我们不提供该载体,载体信息仅供查询和参考。