载体基本信息 | |||
载体名称 | pcDNA1-hFADD-V121N | 载体长度 | 4748 bp |
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载体类型 | Mammalian expression vector | 载体来源 | De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. |
pcDNA1-hFADD-V121N 载体载体图谱
pcDNA1-hFADD-V121N 载体载体序列
LOCUS Exported 4748 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Mammalian expression vector pcDNA1-hFADD-V121N, complete sequence. ACCESSION LT727123 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4748) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 4748) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 4748) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4748 /organism="Mammalian expression vector pcDNA1-hFADD-V121N" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4607." /db_xref="taxon:1944921" rep_origin 25..160 /label=SV40 ori /note="SV40 origin of replication" rep_origin complement(36..111) /direction=LEFT /label=SV40 ori /note="SV40 ori" rep_origin complement(262..374) /direction=LEFT /label=polyoma ori /note="polyoma ori" polyA_signal 1006..1140 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" regulatory complement(1066..1162) /regulatory_class="terminator" /note="SV40 polyA early" regulatory 1066..1162 /regulatory_class="terminator" /note="SV40 polyA late" misc_feature complement(1163..1561) /label=3' UTR of SV40 small t-antigen /note="3' UTR of SV40 small t-antigen" CDS complement(1412..1432) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_feature complement(1562..1628) /label=intron of SV40 small t-antigen /note="intron of SV40 small t-antigen" intron 1562..1627 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS complement(1629..1703) /codon_start=1 /note="unnamed protein product; part of SV40 small t-antigen" /protein_id="SJL87512.1" /translation="DLCEGTLLLWCDIIGQTTYRDLKL" promoter 1711..1729 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" regulatory 1711..1727 /regulatory_class="promoter" /note="SP6 promoter" misc_feature complement(1871..1909) /label=E-tag /note="E-tag" CDS complement(1919..2542) /codon_start=1 /note="unnamed protein product; hFADD mutant" /protein_id="SJL87513.1" /translation="MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDL FSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVI CDNVGKDWRRLARQLKNSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVG ALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS" misc_feature complement(2180..2185) /label=V121N /note="V121N" promoter complement(2561..2579) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory complement(2563..2579) /regulatory_class="promoter" /note="T7 promoter" regulatory complement(2624..3207) /regulatory_class="promoter" /note="hCMV-IE promoter and enhancer" promoter complement(2624..2827) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" enhancer 2828..3207 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" misc_feature complement(3362..3564) /label=supF /note="supF" tRNA complement(3366..3545) /product="E. coli amber-suppressor tyrosine tRNA" /label=supF rep_origin 3688..4146 /label=M13 ori /note="M13 ori" rep_origin complement(4165..4397) /direction=LEFT /label=pMB1 ori /note="pMB1 ori"
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