下载GeneBank文件(.gb)

LOCUS       40924_9111        4623 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Plant transformation vector pCB301 plasmid pXT1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Tao X, Yao M, Hu Z, Wei Q, Feng Z.
  TITLE     Construction of Agrobacterium-mediated Cucumber Mosaic Virus 
            Infectious cDNA Clones and 2b Deletion Viral Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Tao X, Yao M, Hu Z, Wei Q, Feng Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011) Department of Plant Pathology, Nanjing 
            Agricultural University, No.1 Weigang Street, Nanjing, Jiangsu 
            210095, P.R.China
REFERENCE   3  (bases 1 to 4623)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4623)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAY-2011) Department of Plant Pathology, Nanjing Agricultural 
            University, No.1 Weigang Street, Nanjing, Jiangsu 210095, P.R.China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        95..767
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    768..800
                     /label=multiple cloning site region
                     /note="multiple cloning site region"
     ncRNA           800..887
                     /product="HDV-RZ"
                     /label=ribozyme
                     /note="from hepatitis delta virus"
                     /ncRNA_class="ribozyme"
     terminator      898..1150
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1198..1222
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(1377..2522)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2824..3615)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(3827..4458)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4536..4560
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"