载体基本信息 | |||
载体名称 | LIC-pIVEX-LC2 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5236 bp | 载体类型 | Expression vector |
载体来源 | Dortay H, Akula UM, Westphal C, Sittig M, Mueller-Roeber B. |
LIC-pIVEX-LC2 载体载体图谱
LIC-pIVEX-LC2 载体载体序列
LOCUS Exported 5236 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Expression vector LIC-pIVEX-LC2, complete sequence. ACCESSION JF327845 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5236) AUTHORS Dortay H, Akula UM, Westphal C, Sittig M, Mueller-Roeber B. TITLE High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection JOURNAL PLoS ONE 6 (4), E18900 (2011) PUBMED 21541323 REFERENCE 2 (bases 1 to 5236) AUTHORS Dortay H, Mueller-Roeber B. TITLE Direct Submission JOURNAL Submitted (12-FEB-2011) Molecular Biology, University of Potsdam, Germany, Karl-Liebknecht-Str. 24-25, Haus 20, Potsdam, Brandenburg 14476, Germany REFERENCE 3 (bases 1 to 5236) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5236 /organism="Expression vector LIC-pIVEX-LC2" /mol_type="other DNA" /db_xref="taxon:1029638" primer_bind 381..397 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 620..638 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 620..636 /regulatory_class="promoter" /note="T7 promoter" RBS 670..692 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" regulatory 686..691 /regulatory_class="ribosome_binding_site" misc_feature 699..701 /label=start codon /note="start codon" misc_feature 711..718 /label=PmeI recognition site /note="PmeI recognition site" misc_feature 719..1385 /label=LIC stuffer fragment /note="LIC stuffer fragment" misc_feature 1386..1393 /label=PmeI recognition site /note="PmeI recognition site" misc_feature 1399..1419 /label=TEV recognition site /note="TEV recognition site" CDS 1399..1419 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" misc_feature 1429..2412 /label=infrared fluorescent protein /note="infrared fluorescent protein" CDS 1429..2412 /codon_start=1 /product="bacteriophytochrome-based monomeric infrared fluorescent protein (Shu et al., 2009)" /label=IFP1.4 /translation="MARDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT VRELTGFDRVMLYKFAPDATGEMIAEARREGMQAFLGHRFPASHTPAQARALYTRHLLR LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG QLWGLIVCHHQTPYVLPPDLRTTLEELGRKLSGQVQRKEAGMDELYK" misc_feature 2428..2448 /label=6xHis tag /note="6xHis tag" CDS 2428..2445 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" misc_feature 2446..2451 /label=double stop codon (TAA TAA) /note="double stop codon (TAA TAA)" terminator 2553..2600 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" regulatory 2560..2597 /regulatory_class="terminator" /note="T7 terminator" promoter complement(2966..2994) /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" primer_bind complement(3015..3031) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 3039..3055 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3063..3093) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3108..3129 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin 3358..4031 /label=pBR322 origin /note="pBR322 origin" rep_origin complement(3417..4005) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4176..5036) /codon_start=1 /gene="ampR" /product="beta-lactamase" /label=ampR /protein_id="AEE81069.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene complement(4176..5036) /gene="ampR" /label=ampR promoter complement(5037..5141) /gene="bla" /label=AmpR promoter
我们不提供该载体,载体信息仅供查询和参考。