载体基本信息 | |||
载体名称 | pEGFP-N2 | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 4737 bp | 载体类型 | Fluorescent Protein Genes & Plasmids |
载体来源 | Clontech | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pEGFP-N2 载体载体图谱
pEGFP-N2 载体载体序列
LOCUS 40924_17199 4737 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for fusing EGFP to the C-terminus of a partner protein. For other reading frames, use pEGFP-N1 or pEGFP-N3. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4737) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 4737) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4737 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 591..670 /label=MCS /note="multiple cloning site" CDS 683..1399 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" polyA_signal 1525..1646 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(1653..2108) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2135..2239 /label=AmpR promoter promoter 2241..2598 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2633..3424 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3659..3706 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4035..4623 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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