载体基本信息 | |||
载体名称 | pFastBac1 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4776 bp | 载体类型 | Insect Cell Vectors |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pFastBac1 载体载体图谱
pFastBac1 载体载体序列
LOCUS Exported 4776 bp ds-DNA circular SYN 04-4-2014 DEFINITION Vector for high-level expression of proteins in insect cells using the Bac-to-Bac(R) baculovirus system. ACCESSION . VERSION . KEYWORDS pFastBac1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4776) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission COMMENT Transform into DH10Bac(TM) E. coli cells. FEATURES Location/Qualifiers source 1..4776 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" rep_origin 2..457 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 484..588 /gene="bla" /note="AmpR promoter" CDS 589..1449 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1620..2208 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" mobile_element 2511..2735 /mobile_element_type="transposon:Tn7" /note="Tn7R" /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2802..3335) /codon_start=1 /gene="aacC1" /allele=" " /product="gentamycin acetyltransferase" /note="GmR" /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3524..3552) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" promoter 3904..3995 /gene="polh from Autographa californica" /note="polyhedrin promoter" /note="promoter for the baculovirus polyhedrin gene" misc_feature 4032..4142 /note="MCS" /note="multiple cloning site" polyA_signal 4266..4400 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" mobile_element 4429..4594 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)"
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