pFastBac1 载体 (V011648#) COA报告 测序报告

载体基本信息
载体名称 pFastBac1 载体抗性 Ampicillin
载体长度 4776 bp 载体类型 Insect Cell Vectors
载体来源 Invitrogen (Life Technologies) 拷贝数 High copy number

pFastBac1 载体载体图谱

pFastBac14776 bp600120018002400300036004200f1 oriAmpR promoterAmpRoriTn7RGmRPc promoterpolyhedrin promoterMCSSV40 poly(A) signalTn7L

pFastBac1 载体载体序列

LOCUS       Exported                4776 bp ds-DNA    circular SYN 04-4-2014
DEFINITION  Vector for high-level expression of proteins in insect cells using 
            the Bac-to-Bac(R) baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    pFastBac1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
COMMENT     Transform into DH10Bac(TM) E. coli cells.
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     rep_origin      2..457
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /note="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /allele="
                     "
                     /product="gentamycin acetyltransferase"
                     /note="GmR"
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3524..3552)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /note="Pc promoter"
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    4032..4142
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    4266..4400
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     mobile_element  4429..4594
                     /mobile_element_type="transposon:Tn7"
                     /note="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"

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