载体基本信息 | |||
载体名称 | pIRES2-EGFP | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 5308 bp | 载体类型 | Mammalian Expression Vectors |
载体来源 | Clontech | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pIRES2-EGFP 载体载体图谱
pIRES2-EGFP 载体载体序列
LOCUS 40924_25681 5308 bp DNA circular SYN 01-JAN-1980 DEFINITION IRES-containing bicistronic vector for expressing a gene together with EGFP. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5308) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 5308) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT The gene inserted into the MCS should contain start and stop codons. FEATURES Location/Qualifiers source 1..5308 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 600..665 /label=MCS /note="multiple cloning site" misc_feature 667..1253 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 1254..1970 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" polyA_signal 2096..2217 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2224..2679) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2706..2810 /label=AmpR promoter promoter 2812..3169 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 3204..3995 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 4230..4277 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4606..5194 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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