pCDFDuet-1 载体 (V011038#) COA报告 测序报告

载体名称:
pCDFDuet-1
载体抗性:
Streptomycin
载体长度:
3781 bp
载体类型:
pET & Duet Vectors (Novagen)
复制子:
CloDF13 ori
载体来源:
Novagen (EMD Millipore)
拷贝数:
High copy number

pCDFDuet-1 载体载体图谱

pCDFDuet-13781 bp60012001800240030003600lac operatorRBSATG6xHisT7 promoterlac operatorRBSATGS-TagT7 terminatorSmRAmpR promoterCloDF13 oriCAP binding sitelacIlacI promoter

pCDFDuet-1 载体载体序列

LOCUS       pCDFDuet-1.        3781 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector with a CloDF13 (CDF) origin for the co-expression 
            of two genes.
ACCESSION   .
VERSION     .
KEYWORDS    pCDFDuet-1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             71..73
                     /codon_start=1
                     /label=ATG
                     /note="ATG"
                     /translation="M"
     CDS             83..100
                     /label=6xHis
                     /note="6xHis affinity tag"
     promoter        214..232
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    233..257
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             286..291
                     /note="ribosome binding site"
     CDS             300..302
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             366..410
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     terminator      462..509
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(683..1471)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(1472..1563)
                     /label=AmpR promoter
     rep_origin      complement(1611..2349)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(2525..2546)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2562..3641)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3642..3719)
                     /label=lacI promoter

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