载体基本信息 | |||
载体名称 | pCOLADuet-1 | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 3719 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pCOLADuet-1 载体载体图谱
pCOLADuet-1 载体载体序列
LOCUS pCOLADuet-1. 3719 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector with a ColA origin for the co-expression of two genes. ACCESSION . VERSION . KEYWORDS pCOLADuet-1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3719) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 3719) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3719 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 3..27 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 42..64 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 71..73 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 83..100 /label=6xHis /note="6xHis affinity tag" promoter 214..232 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 233..257 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 286..291 /note="ribosome binding site" CDS 300..302 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 366..410 /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" terminator 462..509 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(742..1554) /label=KanR /note="aminoglycoside phosphotransferase" promoter complement(1555..1646) /label=AmpR promoter rep_origin complement(1664..2299) /direction=LEFT /label=ColA ori /note="Plasmids containing the ColA origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." protein_bind complement(2463..2484) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(2500..3579) /label=lacI /note="lac repressor" promoter complement(3580..3657) /label=lacI promoter
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