下载GeneBank文件(.gb)

LOCUS       pET-14b.        4671 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expression of N-terminally 6xHis-tagged 
            proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pET-14b.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     terminator      complement(404..451)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    510..526
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(527..544)
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             complement(554..571)
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             complement(581..583)
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     RBS             complement(590..612)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(644..662)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2223..2411
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2516..2658
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2844..3432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3606..4463)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4464..4568)
                     /label=AmpR promoter