载体基本信息 | |||
载体名称 | pET-15b | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5708 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-15b 载体载体图谱
pET-15b 载体载体序列
LOCUS pET-15b. 5708 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for inducible expression of N-terminally 6xHis-tagged proteins. ACCESSION . VERSION . KEYWORDS pET-15b. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5708) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5708) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5708 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..38 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" terminator complement(213..260) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_feature 319..335 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(336..353) /label=thrombin site /note="thrombin recognition and cleavage site" CDS complement(363..380) /label=6xHis /note="6xHis affinity tag" CDS complement(390..392) /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" RBS complement(399..421) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(436..460) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(461..479) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 788..865 /label=lacI promoter CDS 866..1945 /label=lacI /note="lac repressor" protein_bind 1961..1982 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 3260..3448 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 3553..3695 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3881..4469) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4643..5500) /label=AmpR /note="beta-lactamase" promoter complement(5501..5605) /label=AmpR promoter
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