pET-17b 载体图谱和序列

载体名称:: pET-17b

载体抗性:: Ampicillin

载体长度:: 3306 bp

载体类型:: pET & Duet Vectors (Novagen)

复制子:: ori

载体来源:: Novagen (EMD Millipore)

拷贝数:: High copy number

pET-17b 载体载体图谱

pET-17b 载体载体序列

Copy Sequence

下载GeneBank文件(.gb)

LOCUS       pET-17b.        3306 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expression of N-terminally T7-tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pET-17b.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..75)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    141..228
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(237..269)
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     RBS             complement(277..299)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(331..349)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             858..1046
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1151..1293
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1479..2067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2241..3098)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3099..3203)
                     /label=AmpR promoter

我们不提供该载体，载体信息仅供查询和参考。