| 载体基本信息 | |||
| 载体名称 | pET-24c(+) | 载体抗性 | Kanamycin |
|---|---|---|---|
| 载体长度 | 5308 bp | 载体类型 | pET & Duet Vectors (Novagen) |
| 载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-24c(+) 载体载体图谱
pET-24c(+) 载体载体序列
LOCUS pET-24c(+). 5308 bp DNA circular SYN 01-JAN-1980
DEFINITION Bacterial vector with a kanamycin resistance marker for inducible
expression of T7-tagged proteins. For other reading frames, use
pET-24a(+) or pET-24b(+).
ACCESSION .
VERSION .
KEYWORDS pET-24c(+).
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5308)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5308)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5308
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(26..73)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(140..157)
/label=6xHis
/note="6xHis affinity tag"
misc_feature 158..203
/label=MCS
/note="MCS"
/note="multiple cloning site"
CDS complement(205..237)
/label=T7 tag (gene 10 leader)
/note="leader peptide from bacteriophage T7 gene 10"
RBS complement(245..267)
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
protein_bind complement(282..306)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(307..325)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 634..711
/label=lacI promoter
CDS 712..1791
/label=lacI
/note="lac repressor"
protein_bind 1807..1828
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 2603..2791
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 2896..3038
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(3224..3812)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 3934..4746
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin complement(4842..5297)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
我们不提供该载体，载体信息仅供查询和参考。