- 载体名称:
- pQE-60
- 载体抗性:
- Ampicillin
- 载体长度:
- 3431 bp
- 载体类型:
- Qiagen Vectors
- 复制子:
- ori
- 载体来源:
- Qiagen
- 拷贝数:
- High copy number
- 启动子:
- T5
pQE-60 载体载体图谱
pQE-60 载体载体序列
LOCUS pQE-60. 3431 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing C-terminally 6xHis-tagged proteins, with an NcoI site at the start codon. ACCESSION . VERSION . KEYWORDS pQE-60. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3431) AUTHORS Qiagen TITLE Direct Submission REFERENCE 2 (bases 1 to 3431) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT To use the start codon from a coding sequence, insert at NcoI. FEATURES Location/Qualifiers source 1..3431 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..54 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 62..78 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 101..106 /note="ribosome binding site" misc_feature 113..132 /label=MCS /note="MCS" /note="multiple cloning site" CDS 115..117 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 133..150 /label=6xHis /note="6xHis affinity tag" misc_feature 157..167 /label=stop codons /note="stop codons" /note="stop codons in all three reading frames" terminator 173..267 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 1039..1125 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" misc_feature 1281..1421 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(1607..2195) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2369..3226) /label=AmpR /note="beta-lactamase" promoter complement(3227..3331) /label=AmpR promoter
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