载体基本信息 | |||
载体名称 | pEG202 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 10166 bp | 载体类型 | Yeast Plasmids |
载体来源 | Clontech | 拷贝数 | High copy number |
pEG202 载体载体图谱
pEG202 载体载体序列
LOCUS pEG202. 10166 bp DNA circular SYN 01-JAN-1980 DEFINITION Episomal yeast two-hybrid vector for constitutive expression of LexA fused to the N-terminus of a protein. Also known as pLexA. ACCESSION . VERSION . KEYWORDS pEG202. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10166) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 10166) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..10166 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 807..1511 /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" CDS 1538..2143 /label=LexA /note="E. coli DNA-binding protein that represses genes involved in the SOS response to DNA damage" misc_feature 2144..2182 /label=MCS /note="MCS" /note="multiple cloning site" terminator 2209..2396 /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" rep_origin 3128..4470 /label=2u ori /note="yeast 2u plasmid origin of replication" CDS complement(6117..6773) /label=HIS3 /note="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" promoter complement(6774..6961) /label=HIS3 promoter promoter 8026..8130 /label=AmpR promoter CDS 8131..8988 /label=AmpR /note="beta-lactamase" rep_origin 9162..9750 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(9936..10076) /label=bom /note="basis of mobility region from pBR322"
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