载体基本信息 | |||
载体名称 | pESC-HIS | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6706 bp | 载体类型 | Yeast Plasmids |
载体来源 | Agilent Technologies | 拷贝数 | High copy number |
pESC-HIS 载体载体图谱
pESC-HIS 载体载体序列
LOCUS 40924_17601 6706 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pESC-HIS, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6706) AUTHORS Lu Q. TITLE pESC-HIS JOURNAL Unpublished REFERENCE 2 (bases 1 to 6706) AUTHORS Lu Q. TITLE Direct Submission JOURNAL Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA REFERENCE 3 (bases 1 to 6706) TITLE Direct Submission REFERENCE 4 (bases 1 to 6706) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6706 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 317..503 /label=HIS3 promoter CDS 504..1160 /codon_start=1 /label=HIS3 /note="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM" rep_origin complement(1423..1878) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" terminator complement(1961..2126) /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" CDS complement(2274..2297) /codon_start=1 /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /translation="DYKDDDDK" promoter 2344..3008 /label=GAL1,10 promoter /note="divergent inducible promoter, regulated by Gal4" promoter 3019..3037 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3055..3084 /codon_start=1 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" /translation="EQKLISEEDL" terminator 3114..3303 /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin complement(3546..4134) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4308..5165) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5166..5270) /label=AmpR promoter rep_origin complement(5297..6639) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication"
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