pESC-TRP 载体 (V010320#) COA报告 测序报告

The pESC-TRP is a yeast episomal vector with a TRP1 marker, for galactose-regulated expression and tagging of up to two genes.

载体基本信息
载体名称 pESC-TRP 载体抗性 Ampicillin
载体长度 6525 bp 载体类型 Yeast Plasmids
载体来源 Agilent Technologies 拷贝数 High copy number

pESC-TRP 载体载体图谱

pESC-TRP6525 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300TRP1 promoterTRP1f1 oriADH1 terminatorFLAGGAL1,10 promoterT7 promoterMycCYC1 terminatororiAmpRAmpR promoter2u ori

pESC-TRP 载体载体序列

LOCUS       40924_17611        6525 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pESC-TRP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Lu Q.
  TITLE     pESC-TRP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 6525)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        187..467
                     /label=TRP1 promoter
     CDS             468..1139
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(1779..1944)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2092..2115)
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        2162..2826
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     promoter        2837..2855
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2873..2902
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     terminator      2932..3121
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3364..3952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4126..4983)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4984..5088)
                     /label=AmpR promoter
     rep_origin      complement(5115..6457)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"

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