The pESC-TRP is a yeast episomal vector with a TRP1 marker, for galactose-regulated expression and tagging of up to two genes.
载体基本信息 | |||
载体名称 | pESC-TRP | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6525 bp | 载体类型 | Yeast Plasmids |
载体来源 | Agilent Technologies | 拷贝数 | High copy number |
pESC-TRP 载体载体图谱
pESC-TRP 载体载体序列
LOCUS 40924_17611 6525 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pESC-TRP, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6525) AUTHORS Lu Q. TITLE pESC-TRP JOURNAL Unpublished REFERENCE 2 (bases 1 to 6525) AUTHORS Lu Q. TITLE Direct Submission JOURNAL Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA REFERENCE 3 (bases 1 to 6525) TITLE Direct Submission REFERENCE 4 (bases 1 to 6525) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6525 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 187..467 /label=TRP1 promoter CDS 468..1139 /codon_start=1 /label=TRP1 /note="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" rep_origin complement(1241..1696) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" terminator complement(1779..1944) /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" CDS complement(2092..2115) /codon_start=1 /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /translation="DYKDDDDK" promoter 2162..2826 /label=GAL1,10 promoter /note="divergent inducible promoter, regulated by Gal4" promoter 2837..2855 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2873..2902 /codon_start=1 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" /translation="EQKLISEEDL" terminator 2932..3121 /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin complement(3364..3952) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4126..4983) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4984..5088) /label=AmpR promoter rep_origin complement(5115..6457) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication"
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