| 载体基本信息 | |||
| 载体名称 | pFA6a-5FLAG-kanMX6 | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 4312 bp | 载体类型 | Yeast Plasmids |
| 载体来源 | Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. | 拷贝数 | High copy number |
pFA6a-5FLAG-kanMX6 载体载体图谱
pFA6a-5FLAG-kanMX6 载体载体序列
LOCUS 40924_19216 4312 bp DNA circular SYN 18-DEC-2018
DEFINITION Tagging vector pFA6a-5FLAG-kanMX6, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4312)
AUTHORS Noguchi C, Garabedian MV, Malik M, Noguchi E.
TITLE A vector system for genomic FLAG epitope-tagging in
Schizosaccharomyces pombe
JOURNAL Biotechnol J 3 (9-10), 1280-1285 (2008)
PUBMED 18729046
REFERENCE 2 (bases 1 to 4312)
AUTHORS Noguchi C, Garabedian M, Malik M, Noguchi E.
TITLE Direct Submission
JOURNAL Submitted (16-FEB-2008) Biochemistry and Molecular Biology, Drexel
University College of Medicine, 245 N 15th Street, MS 497,
Philadelphia, PA 19102, USA
REFERENCE 3 (bases 1 to 4312)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4312)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
J 3 (9-10), 1280-1285 (2008)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-FEB-2008) Biochemistry and Molecular Biology, Drexel University
College of Medicine, 245 N 15th Street, MS 497, Philadelphia, PA
19102, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4312
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 69..200
/codon_start=1
/label=5xFLAG
/note="five tandem FLAG(R) epitope tags"
/translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
terminator 227..414
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
gene 489..1845
/label=kanMX
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter complement(1950..1968)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(2226..2814)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2988..3845)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3846..3950)
/label=AmpR promoter
promoter 4296..4312
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
我们不提供该载体，载体信息仅供查询和参考。