pGADT7-Rec 载体 (V010277#)

载体基本信息
载体名称 pGADT7-Rec 载体抗性 Ampicillin
载体长度 8058 bp 载体类型 Yeast Plasmids
载体来源 Clontech 拷贝数 High copy number

pGADT7-Rec 载体载体图谱

pGADT7-Rec8058 bp400800120016002000240028003200360040004400480052005600600064006800720076008000ADH1 promoterATGSV40 NLSGAL4 activation domainT7 promoterATGHASMART IIICDS IIIADH1 terminatorLEU2LEU2 promoterM13 revlac operatorlac promoterCAP binding siteloxPoriAmpRAmpR promoter2u ori

pGADT7-Rec 载体载体序列

LOCUS       pGADT7-Rec.        8058 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High-copy yeast vector for fusing a cDNA to the GAL4 activation 
            domain by in vivo recombination.
ACCESSION   .
VERSION     .
KEYWORDS    pGADT7-Rec.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize with SmaI.
FEATURES             Location/Qualifiers
     source          1..8058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1491..1493
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1521..1541
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1557..1898
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1935..1937
                     /codon_start=1
                     /product="in vitro start codon"
                     /label=in vitro start codon
                     /note="ATG"
                     /translation="M"
     CDS             1941..1967
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    2000..2035
                     /label=SMART III Oligonucleotide Sequence
                     /note="SMART III"
                     /note="matches the SMART III Oligonucleotide for cDNA
                     synthesis"
     misc_feature    2046..2070
                     /label=CDS III Sequence
                     /note="CDS III"
                     /note="matches the CDS III Primer for cDNA synthesis"
     terminator      2486..2673
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2793..3884)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(3885..4290)
                     /label=LEU2 promoter
     primer_bind     complement(4332..4348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4356..4372)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4380..4410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4425..4446)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     protein_bind    4501..4534
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(4885..5473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5647..6504)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6505..6609)
                     /label=AmpR promoter
     rep_origin      6891..8055
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"

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