载体基本信息 | |||
载体名称 | pGADT7-Rec | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 8058 bp | 载体类型 | Yeast Plasmids |
载体来源 | Clontech | 拷贝数 | High copy number |
pGADT7-Rec 载体载体图谱
pGADT7-Rec 载体载体序列
LOCUS pGADT7-Rec. 8058 bp DNA circular SYN 01-JAN-1980 DEFINITION High-copy yeast vector for fusing a cDNA to the GAL4 activation domain by in vivo recombination. ACCESSION . VERSION . KEYWORDS pGADT7-Rec. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8058) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 8058) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Linearize with SmaI. FEATURES Location/Qualifiers source 1..8058 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 771..1475 /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" CDS 1491..1493 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 1521..1541 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" CDS 1557..1898 /label=GAL4 activation domain /note="activation domain of the GAL4 transcriptional activator" promoter 1904..1922 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1935..1937 /codon_start=1 /product="in vitro start codon" /label=in vitro start codon /note="ATG" /translation="M" CDS 1941..1967 /label=HA /note="HA (human influenza hemagglutinin) epitope tag" misc_feature 2000..2035 /label=SMART III Oligonucleotide Sequence /note="SMART III" /note="matches the SMART III Oligonucleotide for cDNA synthesis" misc_feature 2046..2070 /label=CDS III Sequence /note="CDS III" /note="matches the CDS III Primer for cDNA synthesis" terminator 2486..2673 /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" CDS complement(2793..3884) /label=LEU2 /note="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" promoter complement(3885..4290) /label=LEU2 promoter primer_bind complement(4332..4348) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4356..4372) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4380..4410) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4425..4446) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." protein_bind 4501..4534 /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." rep_origin complement(4885..5473) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5647..6504) /label=AmpR /note="beta-lactamase" promoter complement(6505..6609) /label=AmpR promoter rep_origin 6891..8055 /label=2u ori /note="yeast 2u plasmid origin of replication"
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