The pPICZα A is a Pichia pastoris vector for methanol-inducible expression of a secreted protein.
| 载体基本信息 | |||
| 载体名称 | pPICZα A | 载体抗性 | Bleomycin |
|---|---|---|---|
| 载体长度 | 3593 bp | 载体类型 | Yeast Plasmids |
| 载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pPICZα A 载体载体图谱
pPICZα A 载体载体序列
LOCUS pPICZ-A. 3593 bp DNA circular SYN 13-SEP-2021
DEFINITION Pichia pastoris vector for methanol-inducible expression of a
secreted protein.
ACCESSION .
VERSION .
KEYWORDS pPICZ-A.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3593)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 3593)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 3593)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT SGRef: number: 2; type: "Journal Article"
COMMENT Linearize with SacI, PmeI, or BstXI for integration at the AOX1
locus.
FEATURES Location/Qualifiers
source 1..3593
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 2..940
/label=AOX1 promoter
/note="inducible promoter, regulated by methanol"
CDS 941..1207
/label=alpha-factor secretion signal
/note="N-terminal secretion signal from S. cerevisiae
alpha-factor"
misc_feature 1208..1276
/label=MCS
/note="MCS"
/note="MCS;multiple cloning site"
CDS 1275..1304
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 1320..1337
/label=6xHis
/note="6xHis affinity tag"
terminator 1417..1663
/label=AOX1 terminator
/note="transcription terminator for AOX1"
promoter 1678..2089
/label=TEF1 promoter
/note="promoter for EF-1-alpha"
promoter 2097..2144
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 2163..2534
/label=BleoR
/note="antibiotic-binding protein"
terminator 2603..2850
/label=CYC1 terminator
/note="transcription terminator for CYC1"
rep_origin complement(2925..3513)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
我们不提供该载体，载体信息仅供查询和参考。